Cellular cation transport studied by 6, 7Li and 23Na NMR in a porous Mo132 Keplerate type nano‐capsule as model system
Cellular cation transport studied by 6, 7Li and 23Na NMR in a porous Mo132 Keplerate type nano‐capsule as model system
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DOI：
10.1002/mrc.2343
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发表时间：
2008
影响因子：
2
通讯作者：
D. Rehder;E. Haupt;A. Müller
中科院分区：
文献类型：
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作者：
D. Rehder;E. Haupt;A. Müller
Li+离子可以与细胞内外空间中固有存在的其他阳离子（即Na+、K+、Mg2+和Ca2+）相互作用，并具有治疗作用（例如治疗双相情感障碍）或毒性作用（在较高剂量下），可能是因为Li+干扰了上述生理相关阳离子的细胞内/外浓度梯度。细胞跨膜运输可以通过基于氧化钼的 Keplerates 进行建模，即含有 132 个 Mo 中心的纳米级多孔胶囊，通过 6/7Li 以及 23Na NMR 光谱进行监测。报告了水量、温度的变化以及添加有机阳离子“塞子”和移动试剂 [Dy(PPP)2]7− 对 Li+ 阳离子通过这些模型电池“离子通道”运输的影响。在所研究的溶剂系统中，水充当 Li+ 的传输介体。同样，反向传输（Li+/Na+、Li+/K+、Li+/Cs+ 和 Li+/Ca2+）已通过 7Li NMR 进行了研究，在 Li+/Na+ 交换的情况下，通过 23Na NMR 进行了研究，结果表明，大多数（在 Na+ 和 K+ 的情况下）、所有 (Ca2+) 或几乎没有 (Cs+) 的 Li 阳离子从人造细胞的内部位点被挤出到细胞内部。细胞外培养基，而 Na+、K+ 和 Ca2+ 被部分掺入。版权所有 © 2008 约翰·威利父子有限公司
Li+ ions can interplay with other cations intrinsically present in the intra‐ and extra‐cellular space (i.e. Na+, K+, Mg2+ and Ca2+) and have therapeutic effects (e.g. in the treatment of bipolar disorder) or toxic effects (at higher doses), likely because Li+ interferes with the intra‐/extra‐cellular concentration gradients of the mentioned physiologically relevant cations. The cellular transmembrane transport can be modelled by molybdenum‐oxide‐based Keplerates, i.e. nano‐sized porous capsules containing 132 Mo centres, monitored through 6/7Li as well as 23Na NMR spectroscopy. The effects on the transport of Li+ cations through the ‘ion channels’ of these model cells, caused by variations in water amount, temperature, and by the addition of organic cationic ‘plugs’ and the shift reagent [Dy(PPP)2]7− are reported. In the investigated solvent systems, water acts as a transport mediator for Li+. Likewise, the counter‐transport (Li+/Na+, Li+/K+, Li+/Cs+ and Li+/Ca2+) has been investigated by 7Li NMR and, in the case of Li+/Na+ exchange, by 23Na NMR, and it has been shown that most (in the case of Na+ and K+), all (Ca2+) or almost none (Cs+) of the Li cations is extruded from the internal sites of the artificial cell to the extra‐cellular medium, while Na+, K+ and Ca2+ are partially incorporated. Copyright © 2008 John Wiley & Sons, Ltd.