NITRIC-OXIDE PRODUCED BY ACTIVATED ASTROCYTES RAPIDLY AND REVERSIBLY INHIBITS CELLULAR RESPIRATION
NITRIC-OXIDE PRODUCED BY ACTIVATED ASTROCYTES RAPIDLY AND REVERSIBLY INHIBITS CELLULAR RESPIRATION
复制标题
DOI：
10.1016/0304-3940(95)11703-y
复制
发表时间：
1995-07-07
影响因子：
2.5
通讯作者：
CLARK, JB
中科院分区：
文献类型：
--
作者：
BROWN, GC;BOLANOS, JP;CLARK, JB
Cultured astrocytes, activated to express the inducible form of nitric oxide synthase, produced up to 1 mu M nitric oxide (NO) measured by a NO-selective electrode, while non-activated cells produced no detectable NO. The production of NO was associated with an inhibition of cellular respiration, measured simultaneously by an oxygen electrode. The inhibition of respiration was rapidly reversed by inhibiting the NO synthase or by binding the NO with haemoglobin. The respiratory inhibition had an NO, oxygen and substrate dependence consistent with NO-inhibition at cytochrome oxidase. This is the first demonstration that cells can reversibly inhibit mitochondrial respiration via NO production. This inhibition is large and potentially important in a range of pathophysiological conditions.
