Exacerbations of clinical symptoms in human immunodeficiency virus type 1-infected patients with multicentric Castleman's disease are associated with a high increase in Kaposi's sarcoma herpesvirus DNA load in peripheral blood mononuclear cells
Exacerbations of clinical symptoms in human immunodeficiency virus type 1-infected patients with multicentric Castleman's disease are associated with a high increase in Kaposi's sarcoma herpesvirus DNA load in peripheral blood mononuclear cells
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DOI：
10.1086/593567
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发表时间：
1997-05-01
影响因子：
6.4
通讯作者：
Huraux, JM
中科院分区：
文献类型：
--
作者：
Grandadam, M;Dupin, N;Huraux, JM
多中心Castleman病(MCD)和卡波西肉瘤(KS)之间的流行病学联系以及KS疱疹病毒(KSHV)在这两种疾病中的高频率检测提出了这种新病毒在MCD发病机制中所起作用的问题。为了验证这一假说，我们对3例人类免疫缺陷病毒(HIV)感染的MCD患者外周血中蓝染的单个核细胞中FSHV DNA载量进行了检测。临床参数，如发热和淋巴结病的存在，进行了系统的评估。血象和C反应蛋白水平的测定作为标准程序进行。采用半定量聚合酶链式反应方法检测患者外周血单核细胞中KSHV DNA载量。与MCD相关的临床和生物学参数的变化与KSHV DNA载量之间存在相关性，提示HIV感染者中KSHV与MCD密切相关。
The epidemiologic link between multicentric Castleman's disease (MCD) and Kaposi's sarcoma (KS) and the high frequency of KS herpesvirus (KSHV) detection in both diseases raise the question of a role of this new virus in the pathogenesis of MCD. To explore this hypothesis, the FSHV DNA load was investigated in peripheral blued mononuclear cells of 3 human immunodeficiency virus (HIV)-infected patients with MCD at different points during the clinical course. Clinical parameters, such as fever and the presence of lymphadenopathy, were systematically assessed. Hemogram and C-reactive protein level determinations were performed as standard procedures. KSHV DNA load was investigated by means of semiquantitative polymerase chain reaction assay using peripheral blood mononuclear cells of the patients. A correlation between the variation in clinical and biologic parameters related to MCD and KSHV DNA load was found, suggesting a close relationship between KSHV and MCD in HIV-infected patients.