HPCA1 is required for systemic reactive oxygen species and calcium cell-to-cell signaling and plant acclimation to stress
HPCA1 is required for systemic reactive oxygen species and calcium cell-to-cell signaling and plant acclimation to stress
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DOI：
10.1093/plcell/koac241
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发表时间：
2022-08-04
期刊：
影响因子：
11.6
通讯作者：
Mittler, Ron
中科院分区：
文献类型：
--
作者：
Fichman, Yosef;Zandalinas, Sara, I;Mittler, Ron
在植物适应逆境过程中，富含亮氨酸的重复受体样激酶HPCA1介导和协调系统细胞间活性氧和钙信号。活性氧（ROS）是由外质体呼吸爆发氧化酶同源物（RBOHs）产生的，在植物适应逆境所需的局部和系统细胞间信号传导中起关键作用。在这里，我们揭示了拟南芥亮氨酸-富重复受体样激酶h2o2诱导的CA（2+）增加1 （HPCA1）作为一个中心ROS受体，在细胞间ROS信号的传播、响应不同生物和非生物胁迫的系统信号、局部和全身组织的胁迫反应以及植物在局部处理强光（HL）胁迫后对胁迫的适应中所必需的。我们进一步报道了HPCA1是全身钙信号所必需的，而不是全身膜去极化反应所必需的，并确定了钙渗透通道MECHANOSENSITIVE ION channel LIKE 3， CALCINEURIN B-LIKE calcium SENSOR 4 (CBL4)， CBL4- interaction PROTEIN KINASE 26和蔗糖-非发酵-1相关蛋白激酶2.6/OPEN STOMATA 1 （OST1）是细胞间ROS信号传播所必需的。此外，我们发现RBOHD的丝氨酸残基S343和S347 （OST1的假定靶标）在局部应用HL胁迫时细胞间ROS信号传导中发挥关键作用。我们的研究结果表明，HPCA1在介导和协调植物适应胁迫所需的系统细胞间ROS和钙信号中起关键作用。
The leucine-rich-repeat receptor-like kinase HPCA1 functions in mediating and coordinating systemic cell-to-cell reactive oxygen species and calcium signals during plant acclimation to stress.Reactive oxygen species (ROS), produced by respiratory burst oxidase homologs (RBOHs) at the apoplast, play a key role in local and systemic cell-to-cell signaling, required for plant acclimation to stress. Here we reveal that the Arabidopsis thaliana leucine-rich-repeat receptor-like kinase H2O2-INDUCED CA(2+) INCREASES 1 (HPCA1) acts as a central ROS receptor required for the propagation of cell-to-cell ROS signals, systemic signaling in response to different biotic and abiotic stresses, stress responses at the local and systemic tissues, and plant acclimation to stress, following a local treatment of high light (HL) stress. We further report that HPCA1 is required for systemic calcium signals, but not systemic membrane depolarization responses, and identify the calcium-permeable channel MECHANOSENSITIVE ION CHANNEL LIKE 3, CALCINEURIN B-LIKE CALCIUM SENSOR 4 (CBL4), CBL4-INTERACTING PROTEIN KINASE 26 and Sucrose-non-fermenting-1-related Protein Kinase 2.6/OPEN STOMATA 1 (OST1) as required for the propagation of cell-to-cell ROS signals. In addition, we identify serine residues S343 and S347 of RBOHD (the putative targets of OST1) as playing a key role in cell-to-cell ROS signaling in response to a local application of HL stress. Our findings reveal that HPCA1 plays a key role in mediating and coordinating systemic cell-to-cell ROS and calcium signals required for plant acclimation to stress.