Differentiation, maturation, and survival of dendritic cells by osteopontin regulation
Differentiation, maturation, and survival of dendritic cells by osteopontin regulation
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DOI：
10.1128/cdli.12.1.206-212.2005
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发表时间：
2005-01-01
期刊：
影响因子：
--
通讯作者：
Sasaki, Y
中科院分区：
文献类型：
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作者：
Kawamura, K;Iyonaga, K;Sasaki, Y
树突状细胞（dc）是抗原呈递细胞，具有诱导先天性免疫和适应性免疫所必需的初级免疫反应的能力。骨桥蛋白（OPN）是一种含有精氨酸-甘氨酸-天冬氨酸序列的分泌性酸性磷蛋白，已被认为在早期细胞免疫应答中起重要作用。DCs和OPN之间的相互作用尚未明确。我们假设dc和OPN之间存在重要的相互作用，OPN是早期细胞免疫反应中不可或缺的细胞外基质成分。人类单核细胞来源的dc合成OPN，特别是在单核细胞向未成熟dc分化的过程中。通过抗OPN抗体阻断OPN，培养的dc变小，表达的共刺激分子和主要组织相容性复合体II类抗原水平低于未处理的dc。此外，抗opn抗体处理的树突状细胞容易发生凋亡。这些结果表明，人类dc可以产生OPN，并且OPN可能通过自分泌和/或旁分泌途径在dc的分化、成熟和存活中发挥作用。
Dendritic cells (DCs) are antigen-presenting cells with the ability to induce primary immune responses necessary in innate immunity and adaptive immunity. Osteopontin (OPN) is a secreted acidic phosphoprotein containing an arginine-glycine-aspartate sequence and has been suggested to play an important role in early cellular immune responses. The interaction between DCs and OPN has not been clarified. We hypothesized that there is an important interaction between DCs and OPN, which is an indispensable extracellular matrix component in early cellular immune responses. Human monocyte-derived DCs synthesized OPN especially during the differentiation from monocytes to immature DCs. By blocking of OPN with anti-OPN antibody, cultured DCs became smaller and expressed lower levels of costimulatory molecules and major histocompatibility complex class II antigens than untreated DCs. Furthermore, DCs treated with anti-OPN antibody easily underwent apoptosis. These results suggest that human DCs can produce OPN and that OPN may play a role in the differentiation, maturation, and survival of DCs by autocrine and/or paracrine pathways.