Temporal correlation between UV radiation locally inducible tolerance and the sequential appearance of dermal, then epidermal, class II MHC(+)CD11b(+) monocytic/macrophagic cells
Temporal correlation between UV radiation locally inducible tolerance and the sequential appearance of dermal, then epidermal, class II MHC(+)CD11b(+) monocytic/macrophagic cells
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DOI：
10.1111/1523-1747.ep12365802
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发表时间：
1996-11-01
影响因子：
6.5
通讯作者：
Cooper, KD
中科院分区：
文献类型：
--
作者：
Hammerberg, C;Duraiswamy, N;Cooper, KD
我们进行了一项时程研究，以确定接触致敏主动抑制的诱导与紫外线照射的真皮和表皮中涉及局部诱导免疫耐受的各种细胞的存在之间的体内关系：II类主要组织相容性复合体(MHC)(+)CD11b(LO)Gr-1(-)朗格汉斯细胞(LC)，II类MHC(-)CD45(+)CD3(+)树突状表皮T细胞，II类MHC(+)CD11b(+)GR-1(-)单核细胞或II类MHC(+)CD11b(+)GR-1(+)单核/巨噬细胞72mJ/cm紫外线照射后6h即可检测到部分耐受(50%)，紫外线照射后48h可检测到最大耐受，经免疫细胞仪检测，紫外线照射后6h，真皮内低颗粒LC亚群消失，高颗粒朗格汉斯细胞亚群减少较慢，6h真皮内可见少量浸润性单核/巨噬细胞，至紫外线照射后24h，耐受性增加至70%。紫外光照射48h后，真皮中浸润性单核/巨噬细胞数量已上升至真皮细胞总数的1.2%，并首次与其他浸润性白细胞(即中性粒细胞)一起在表皮中被观察到。当达到最大耐受状态时，构成表皮和真皮抗原提呈细胞的数量都处于或接近枯竭的最低点，而浸润性单核/巨噬细胞数量在48h和72h时显示出表皮的急剧增加。局部诱导体内耐受的能力与真皮和表皮中II类MHC(+)、CD11b(+)、Gr-1(+)和单核/巨噬细胞的扩张密切相关。
We performed a time course study in order to define the in vivo relationship between the induction of active suppression of contact sensitization and the presence of various cells in ultraviolet-exposed dermis and epidermis implicated in locally inducible immune tolerance: class II major histocompatibility complex (MHC)(+)CD11b(lo)Gr-1(-) Langerhans cells (LC), class II MHC(-)CD45(+)CD3(+) dendritic epidermal T cells, class II MHC(+)CD11b(+)Gr-1(-) monocytes or class II MHC(+)CD11b(+)Gr-1(+) monocytic/macrophagic cells, Partial tolerance (50%) was first detectable 6 h after a single 72 mJ/cm(2) ultraviolet B exposure and maximum tolerance at 48 h post-ultraviolet exposure, By how cytometry, a low granularity LC subset had disappeared from the epidermis within 6 h after ultraviolet exposure, followed by a slower decrease in the high granularity Langerhans cells subset, Within the dermis at the 6-h time point, small numbers of infiltrating monocytic/macrophagic cells are already apparent, By 24 h post-ultraviolet exposure, at which time tolerance has increased to 70%, the infiltrating monocytic/macrophagic population had risen to 1.2% of the total dermal cell population and was observed for the first time in the epidermis along with other infiltrating leukocytes (i.e., polymorphonuclear leukocytes), By 48 h post-ultraviolet exposure, when a state of maximum tolerance is obtained, both constitutive epidermal and dermal antigen-presenting cell populations were at or near their nadir of depletion, The infiltrating monocyte/macrophage population, however, exhibited a dramatic increase in the epidermis at 48 and 72 h, Thus, the ability to locally induce a state of in vivo tolerance is closely associated with the expansion of class II MHC(+)CD11b(+)Gr-1(+) and monocytic/macrophagic cells in the dermis and epidermis.