Distribution of monoclonal antibodies in intestinal and urogenital secretions of mice bearing hybridoma 'backpack' tumours.
Distribution of monoclonal antibodies in intestinal and urogenital secretions of mice bearing hybridoma 'backpack' tumours.
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携带杂交瘤“背包”肿瘤的小鼠的肠道和泌尿生殖分泌物中单克隆抗体的分布。
DOI：
10.1046/j.1365-3083.1997.d01-383.x
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发表时间：
1997
影响因子：
3.7
通讯作者：
Neutra,MR
中科院分区：
文献类型：
--
作者：
Haneberg,B;Kendall,D;Apter,FM;Neutra,MR
携带伊加杂交瘤“背包”肿瘤的小鼠已被用于证明分泌单个单克隆伊加可保护免受粘膜感染，但该模型与正常伊加保护的相关性尚不清楚。作者分析了带有抗霍乱毒素（CT）伊加和IgG背包肿瘤的小鼠（有和没有胆管结扎）的胆汁、局部肠分泌物、宫颈阴道分泌物、尿液和血清中特异性单克隆抗体和总抗体的分布。背包肿瘤导致血清中的抗CT和总伊加或IgG以及胆汁中的伊加（而非IgG）水平高。通过吸收性过滤器"芯“从背包肿瘤小鼠整个肠道的粘膜表面回收的分泌物含有显著浓度的单克隆抗CT伊加，但总伊加水平与正常小鼠相同。无论是单克隆或总伊加水平粘膜表面上的胆管结扎改变。此外，背包肿瘤小鼠局部肠道分泌物中的抗CT单克隆伊加水平与先前通过CT粘膜免疫引起的特异性多克隆伊加水平相当。因此，在背包肿瘤模型中，伊加介导的对肠道攻击的保护可能是体内天然粘膜免疫提供的保护的有效预测因子。然而，胆汁、尿液和雌性生殖道中的高单克隆伊加水平可能并不反映正常免疫小鼠的情况。
Mice bearing IgA hybridoma ‘backpack’ tumours have been used to demonstrate that secretion of a single monoclonal IgA can protect against mucosal infection, but the relevance of this model to normal IgA protection is not clear. The authors analysed the distribution of specific monoclonal and total antibodies in bile, local intestinal secretions, cervical‐vaginal secretions, urine and serum of mice bearing anti‐cholera toxin (CT) IgA and IgG backpack tumours, with and without bile duct ligation. Backpack tumours resulted in high levels of both anti‐CT and total IgA or IgG in serum, and IgA (but not IgG) in bile. Secretions recovered by absorbent filter ‘wicks’ from mucosal surfaces throughout the intestines of backpack tumour mice contained significant concentrations of monoclonal anti‐CT IgA, but total IgA levels were as in normal mice. Neither monoclonal nor total IgA levels on mucosal surfaces were altered by bile duct ligation. Furthermore, anti‐CT monoclonal IgA levels in local intestinal secretions of backpack tumour mice were comparable to specific polyclonal IgA levels previously elicited by mucosal immunization with CT. Thus, IgA‐mediated protection against enteric challenge in the backpack tumour model may be a valid predictor of protection provided by natural mucosal immunizationin vivo. High monoclonal IgA levels in bile, urine and the female genital tract, however, may not reflect the situation in normal immunized mice.