Comparing the effect of equiaxial cyclic mechanical stimulation on GATA4 expression in adipose-derived and bone marrow-derived mesenchymal stem cells
Comparing the effect of equiaxial cyclic mechanical stimulation on GATA4 expression in adipose-derived and bone marrow-derived mesenchymal stem cells
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DOI：
10.1002/cbin.10194
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发表时间：
2014-02-01
影响因子：
3.9
通讯作者：
Abbasnia, Pegah
中科院分区：
文献类型：
--
作者：
Amin, Susan;Banijamali, Seyede Elnaz;Abbasnia, Pegah
心肌细胞容易受到机械刺激，其中脉动血流对心脏施加径向和纵向应变。最近的研究表明，机械刺激可以显着影响心肌细胞的再生。GATA 4是一种心脏特异性转录因子，在胚胎心脏发育后期起重要作用。本研究旨在研究等轴循环应变对脂肪源性（ASCs）和骨髓源性（BMSCs）间充质干细胞GATA 4表达的影响。出于这个原因，在化学、机械、机械化学和阴性对照的四个不同组中研究了ASC和BMSC。根据该分类，将细胞暴露于循环机械负荷和/或5-氮杂胞苷作为化学因子。然后在第一、第四和第七天使用实时PCR方法定量GATA 4表达水平。结果表明：（1）间充质干细胞的等轴循环刺激可以从诱导的早期促进GATA 4的表达，并且随着其继续，其与化学因子的组合提高表达;（2）与化学因子相比，循环应变可以加速GATA 4的表达;（3）在这方面，这些结果表明ASC比BMSC表达GATA 4的能力更高。
Myocardium is prone to mechanical stimuli among which pulsatile blood flow exerts both radial and longitudinal strains on the heart. Recent studies have shown that mechanical stimulation can notably influence regeneration of cardiac muscle cells. GATA4 is a cardiac-specific transcription factor that plays an important role in late embryonic heart development. Our study aimed at investigating the effect of equiaxial cyclic strain on GATA4 expression in adipose-derived (ASCs) and bone marrow-derived (BMSCs) mesenchymal stem cells. For this reason, both ASCs and BMSCs were studied in four distinct groups of chemical, mechanical, mechano-chemical and negative control. According to this categorisation, the cells were exposed to cyclic mechanical loading and/or 5-azacytidine as the chemical factor. The level of GATA4 expression was then quantified using real-time PCR method on the first, fourth and seventh days. The results show that: (1) equiaxial cyclic stimulation of mesenchymal stem cells could promote GATA4 expression from the early days of induction and as it went on, its combination with chemical factor elevated expression; (2) cyclic strain could accelerate GATA4 expression compared to the chemical factor; (3) in this regard, these results indicate a higher capacity of ASCs than BMSCs to express GATA4.