The papain-like cysteine protease CEP1 is involved in programmed cell death and secondary wall thickening during xylem development in Arabidopsis.
The papain-like cysteine protease CEP1 is involved in programmed cell death and secondary wall thickening during xylem development in Arabidopsis.
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木瓜蛋白酶样半胱氨酸蛋白酶 CEP1 参与拟南芥木质部发育过程中的程序性细胞死亡和次生壁增厚
DOI：
10.1093/jxb/ery356
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发表时间：
2019-01-01
影响因子：
6.9
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Han J;Li H;Yin B;Zhang Y;Liu Y;Cheng Z;Liu D;Lu H
木瓜蛋白酶样半胱氨酸蛋白酶CEP1参与细胞程序性死亡过程中细胞内容物的清除和木质部发育过程中次生壁增厚的调节。在木质部发育过程中，导管分子和纤维细胞都经历程序性细胞死亡（PCD）。在这项研究中，我们研究了木瓜蛋白酶样半胱氨酸蛋白酶CEP1在拟南芥木质部PCD中的作用。CEP1定位于木质部细胞的细胞壁中，并且在花序茎中的表达水平随着茎成熟而增加。与野生型植物相比，CEP1突变体植物表现出延迟的茎生长和减少的木质部细胞数量。透射电子显微镜表明，细胞器降解延迟PCD期间，和较厚的次生壁存在于纤维细胞和导管元件的cep1突变体。花序茎成熟阶段的转录分析显示，参与次生壁成分生物合成的基因，包括纤维素，半纤维素，木质素，以及木材相关的转录因子，在cep1突变体中上调。这些结果表明，CEP1直接参与PCD过程中细胞内容物的清除，并在木质部发育过程中调节次生壁增厚。
The papain-like cysteine protease CEP1 is involved in clearing cellular contents during programmed cell death and regulating secondary wall thickening during xylem development. Both tracheary elements and fiber cells undergo programmed cell death (PCD) during xylem development. In this study we investigated the role of papain-like cysteine protease CEP1 in PCD in the xylem of Arabidopsis. CEP1 was located in the cell wall of xylem cells, and CEP1 expression levels in inflorescence stems increased during stem maturation. cep1 mutant plants exhibited delayed stem growth and reduced xylem cell number compared to wild-type plants. Transmission electron microscopy demonstrated that organelle degradation was delayed during PCD, and thicker secondary walls were present in fiber cells and tracheary elements of the cep1 mutant. Transcriptional analyses of the maturation stage of the inflorescence stem revealed that genes involved in the biosynthesis of secondary wall components, including cellulose, hemicellulose, and lignin, as well as wood-associated transcriptional factors, were up-regulated in the cep1 mutant. These results suggest that CEP1 is directly involved in the clearing of cellular content during PCD and regulates secondary wall thickening during xylem development.
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影响因子：
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通讯作者：
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