Analysis of the nucleotide sequence of the guinea pig cytomegalovirus (GPCMV) genome.
Analysis of the nucleotide sequence of the guinea pig cytomegalovirus (GPCMV) genome.
复制标题
豚鼠巨细胞病毒（GPCMV）基因组的核苷酸序列分析。
DOI：
10.1186/1743-422x-5-139
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发表时间：
2008-11-12
期刊：
影响因子：
4.8
通讯作者：
McVoy MA
中科院分区：
文献类型：
--
作者：
Schleiss MR;McGregor A;Choi KY;Date SV;Cui X;McVoy MA
In this report we describe the genomic sequence of guinea pig cytomegalovirus (GPCMV) assembled from a tissue culture-derived bacterial artificial chromosome clone, plasmid clones of viral restriction fragments, and direct PCR sequencing of viral DNA. The GPCMV genome is 232,678 bp, excluding the terminal repeats, and has a GC content of 55%. A total of 105 open reading frames (ORFs) of > 100 amino acids with sequence and/or positional homology to other CMV ORFs were annotated. Positional and sequence homologs of human cytomegalovirus open reading frames UL23 through UL122 were identified. Homology with other cytomegaloviruses was most prominent in the central ~60% of the genome, with divergence of sequence and lack of conserved homologs at the respective genomic termini. Of interest, the GPCMV genome was found in many cases to bear stronger phylogenetic similarity to primate CMVs than to rodent CMVs. The sequence of GPCMV should facilitate vaccine and pathogenesis studies in this model of congenital CMV infection.
