Temporospatial gene expression and protein localization of matrix metalloproteinases and their inhibitors during mouse molar tooth development
Temporospatial gene expression and protein localization of matrix metalloproteinases and their inhibitors during mouse molar tooth development
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DOI：
10.1002/dvdy.10352
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发表时间：
2003-09
影响因子：
2.5
通讯作者：
N. Yoshiba;K. Yoshiba;C. Stoetzel;F. Perrin‐Schmitt;Y. Cam;J. Ruch;H. Lesot
中科院分区：
文献类型：
--
作者：
N. Yoshiba;K. Yoshiba;C. Stoetzel;F. Perrin‐Schmitt;Y. Cam;J. Ruch;H. Lesot
分析了小鼠磨牙发育过程中基质金属蛋白酶（MMP）-2，9，膜型MMP（MT 1-MMP）以及TIMP-1，-2和-3的基因表达和蛋白分布。免疫组化结果显示，所有研究的MMPs在牙上皮和间充质中均有表达。相反，TIMPs的基因和蛋白表达分析表明，他们的差异表达。TIMP-1在E13和E16之间在牙上皮和间充质中表达，并在E14（帽状期）瞬时上调。TIMP-1在分化的成牙本质细胞中也有表达。TIMP-2 RNA转录物存在于牙周和牙齿间充质、成牙本质细胞和成釉细胞中。蛋白质分析显示，高表达的舌侧的牙齿上皮细胞和下面的间充质连同瞬时表达的釉结在E14和表达的牙龈组织和釉基质出生后。TIMP-3 RNA转录物存在于牙上皮的离散区域，包括E16时宫颈环中的高水平。在E16的前成牙本质细胞中也检测到表达，并且在成釉细胞分化期间短暂表达。蛋白分布分析显示，E14时牙上皮舌侧TIMP-3水平较低。MT 1-MMP在E13和E16之间的牙齿间充质中表达，在E14的颈环中表达相对较高水平，并且在成牙本质细胞和成釉细胞中表达。不同的时空分布模式的TIMPs表明，这些抑制剂在牙齿发育过程中发挥了一些内在的作用。Developmental Dynamics 228：105-112，2003.© 2003 Wiley利斯公司
The gene expression and protein distribution of matrix metalloproteinase (MMP) ‐2, ‐9, membrane type‐1 MMP (MT1‐MMP), as well as of TIMP‐1, ‐2, and ‐3 were analyzed during mouse molar development. Immunohistochemical data demonstrated that all the MMPs investigated were expressed in the dental epithelium and mesenchyme. In contrast, gene and protein expression analysis for TIMPs showed that they were differentially expressed. TIMP‐1 was expressed in the dental epithelium and mesenchyme between E13 and E16 and was transiently up‐regulated at E14, the cap stage. TIMP‐1 expression was also detected in differentiating odontoblasts. TIMP‐2 RNA transcripts were found in the peridental and dental mesenchyme, odontoblasts, and ameloblasts. Protein analysis revealed high expression on the lingual side of the dental epithelium and underlying mesenchyme together with transient expression in the enamel knot at E14 and expression in the gingival tissue and enamel matrix postnatally. TIMP‐3 RNA transcripts were found in discrete regions of the dental epithelium, including at high levels in the cervical loop at E16. Expression was also detected in preodontoblasts at E16 and transiently during ameloblast differentiation. Analysis of the protein distribution revealed a lower level of TIMP‐3 on the lingual side of the dental epithelium at E14. MT1‐MMP was expressed in the dental mesenchyme between E13 and E16, at relatively high levels in the cervical loop at E14, and in the odontoblasts and ameloblasts. The distinct temporospatial distribution patterns of the TIMPs suggest that these inhibitors play several intrinsic roles during tooth development. Developmental Dynamics 228:105–112, 2003. © 2003 Wiley‐Liss, Inc.