The regulation of caveolin expression and localization by serum and heparin in vascular smooth muscle cells.
The regulation of caveolin expression and localization by serum and heparin in vascular smooth muscle cells.
复制标题
血管平滑肌细胞中血清和肝素对小窝蛋白表达和定位的调节。
DOI：
10.1006/bbrc.1999.1738
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Simari,RD
中科院分区：
文献类型：
--
作者：
Peterson,TE;Kleppe,LS;Caplice,NM;Pan,S;Mueske,CS;Simari,RD
Caveolae have been implicated in growth factor receptor and G-protein coupled receptor signaling in vascular cells. It has been postulated that caveolin, the structural protein of caveolae, may act as a general tyrosine kinase inhibitor by binding and inhibiting signaling molecules involved in the activation of the MAP kinase proliferation cascade. Using an in vitro model of VSMC proliferation, we found that serum stimulation caused a dose dependent decrease in both caveolin-1 and caveolin-2 protein levels in human coronary artery smooth muscle cells. Heparin, an inhibitor of VSMC proliferation, inhibited the serum-induced loss of caveolin-1 and caveolin-2. In addition, heparin caused an increase in both caveolin-1 and caveolin-2 localization to caveolae-enriched sucrose gradient membrane fractions when compared to serum alone. Taken together, caveolin may play an important role in the regulation of VSMC proliferation and heparin and serum have opposing effects on caveolin expression and localization in VSMC.
