Metabolic activation of the HOG MAP kinase pathway by Snf1/AMPK regulates lipid signaling at the Golgi.
Metabolic activation of the HOG MAP kinase pathway by Snf1/AMPK regulates lipid signaling at the Golgi.
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DOI：
10.1111/j.1600-0854.2012.01406.x
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Mayinger P
中科院分区：
文献类型：
--
作者：
Piao H;MacLean Freed J;Mayinger P
磷酸磷脂酰肌醇-4-磷酸（PI(4)P）是高尔基体功能的重要调节因子。高尔基体PI(4)P的代谢调节需要在内质网（ER）和高尔基膜之间易位的脂质磷酸酶Sac1。Sac1的定位响应葡萄糖水平的变化，但调节Sac1交通的上游信号通路尚不清楚。在这里，我们报道了丝裂原活化蛋白激酶（MAPK） Hog1将葡萄糖信号传递给高尔基体并调节Sac1的定位。我们发现Hog1在葡萄糖饥饿和葡萄糖刺激下都能快速激活，它独立于对渗透胁迫的反应，但需要上游元件Ssk1，并由amp活化激酶（AMPK）的酵母同源物Snf1控制。消除Hog1或Snf1均可减缓葡萄糖诱导的Sac1脂质磷酸酶从高尔基体向内质网的易位，从而延缓PI(4)P在高尔基体的积累。我们得出结论，在高尔基体脂质信号的代谢控制中，HOG通路和Snf1/AMPK之间需要一种新的串扰。
Phosphatidylinositol-4-phosphate (PI(4)P) is an important regulator of Golgi function. Metabolic regulation of Golgi PI(4)P requires the lipid phosphatase Sac1 that translocates between endoplasmic reticulum (ER) and Golgi membranes. Localization of Sac1 responds to changes in glucose levels, yet the upstream signaling pathways that regulate Sac1 traffic are unknown. Here, we report that mitogen-activated protein kinase (MAPK) Hog1 transmits glucose signals to the Golgi and regulates localization of Sac1. We find that Hog1 is rapidly activated by both glucose starvation and glucose stimulation, which is independent of the well-characterized response to osmotic stress but requires the upstream element Ssk1 and is controlled by Snf1, the yeast homolog of AMP-activated kinase (AMPK). Elimination of either Hog1 or Snf1 slows glucose-induced translocation of Sac1 lipid phosphatase from the Golgi to the ER and thus delays PI(4)P accumulation at the Golgi. We conclude that a novel cross-talk between the HOG pathway and Snf1/AMPK is required for the metabolic control of lipid signaling at the Golgi.
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影响因子：
2.7
作者：
Coccetti, Paola;Montano, Giuseppe;Pagliarin, Roberto
通讯作者：
Pagliarin, Roberto
影响因子：
--
作者：
Nandy SK;Jouhten P;Nielsen J
通讯作者：
Nielsen J
DOI：
10.1083/jcb.201102092
发表时间：
2011-05-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mao K;Wang K;Zhao M;Xu T;Klionsky DJ
通讯作者：
Klionsky DJ
影响因子：
3.5
作者：
Kim, Sungjoon;Shah, Kavita
通讯作者：
Shah, Kavita
影响因子：
7.8
作者：
Anastasia, Steph D.;Duy Linh Nguyen;Kellogg, Douglas R.
通讯作者：
Kellogg, Douglas R.