Cooperation of Aspergillus nidulans enzymes increases plant polysaccharide saccharification.
Cooperation of Aspergillus nidulans enzymes increases plant polysaccharide saccharification.
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构巢曲霉酶的协同作用增加了植物多糖的糖化作用。
DOI：
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发表时间：
2016
影响因子：
4.7
通讯作者：
R. D. de Vries
中科院分区：
文献类型：
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作者：
Robson Tramontina;D. Robl;G. P. Maitan;R. D. de Vries
有效的多糖降解取决于作用于主链和侧链的酶之间的相互作用。以前的研究证明了几种酶之间的合作，但并不是所有的酶组合都被探索过。更好地了解酶的合作将使设计更好的酶混合物，最佳地从协同效应中获益。在这项研究中，我们分析了参与木聚糖，葡聚糖，木葡聚糖和粗植物生物质从构巢曲霉通过单一和组合孵育与他们的聚合物基板的降解的几种酶的合作。在大多数酶之间观察到积极的影响，尽管并不总是达到相同的程度。此外，在本研究中配制的特制鸡尾酒导致葡萄糖从植物生物质中的有效释放。这项研究也为植物生物质糖化中酶之间的复杂合作以及在易于获得的宿主（如巴斯德毕赤酵母）中的表达如何有助于揭示这些效应提供了一个例子。
Efficient polysaccharide degradation depends on interaction between enzymes acting on the main chain and the side chains. Previous studies demonstrated cooperation between several enzymes, but not all enzyme combinations have been explored. A better understanding of enzyme cooperation would enable the design of better enzyme mixtures, optimally profiting from synergistic effects. In this study, we analyzed the cooperation of several enzymes involved in the degradation of xylan, glucan, xyloglucan and crude plant biomass from Aspergillus nidulans by single and combined incubations with their polymeric substrate. Positive effects were observed between most enzymes, although not always to the same extent. Moreover, the tailor made cocktails formulated in this study resulted in efficient release of glucose from plant biomass. This study also serves as an example for the complex cooperation that occurs between enzymes in plant biomass saccharification and how expression in easily-accessible hosts, such as Pichia pastoris, can help in revealing these effects.