Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin
Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin
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DOI：
10.1126/science.283.5408.1739
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发表时间：
1999-03-12
期刊：
影响因子：
56.9
通讯作者：
Akiyama, T
中科院分区：
文献类型：
--
作者：
Hamada, F;Tomoyasu, Y;Akiyama, T
Wnt/Wingless在发育过程中指导许多细胞命运。Wnt/Wingless信号增加β-catenin/Armadillo的量，从而激活基因转录。在这里，果蝇蛋白D-Axin显示与Armadillo和D-APC相互作用。d-axin的突变导致细胞质中Armadillo和Wingless靶基因产物之一Distal-less的积累，d-axin的异位表达抑制Wingless信号传导。因此，D-Axin通过下调Armadillo的水平来负调节Wingless信号传导。这些结果确立了Axin蛋白家族在果蝇中Wnt/Wingless信号传导中的重要性。
Wnt/Wingless directs many cell fates during development. Wnt/Wingless signaling increases the amount of beta-catenin/Armadillo, which in turn activates gene transcription. Here the Drosophila protein D-Axin was shown to interact with Armadillo and D-APC. Mutation of d-axin resulted in the accumulation of cytoplasmic Armadillo and one of the Wingless target gene products, Distal-less. Ectopic expression of d-axin inhibited Wingless signaling. Hence, D-Axin negatively regulates Wingless signaling by down-regulating the Level of Armadillo. These results establish the importance of the Axin family of proteins in Wnt/Wingless signaling in Drosophila.