Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus
Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus
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DOI：
10.1038/nbt0398-253
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发表时间：
1998-03-01
影响因子：
46.9
通讯作者：
Evans, SM
中科院分区：
文献类型：
--
作者：
Fu, YC;Wang, YB;Evans, SM
通过将DNA直接注射到脊椎动物胚胎中，在单代内表达转基因一直受到低效和不均匀基因表达的困扰。我们报道了一种新的策略，通过直接将DNA注射到发育中的非洲爪蟾胚胎中，有效和稳定地表达由普遍存在的和组织特异性启动子驱动的转基因。该策略包括将感兴趣的表达盒与腺相关病毒的反向末端重复序列（ITRs）连接在一起。我们的研究结果表明，ITR策略可能普遍适用于其他系统，如斑马鱼和胚胎干细胞，并可能在有问题的环境中实现转基因的组织特异性表达。
Expression of transgenes within a single generation by direct DNA injection into vertebrate embryos has been plagued by inefficient and nonuniform gene expression. We report a novel strategy for efficient and stable expression of transgenes driven by both ubiquitous and tissue-specific promoters by direct DNA injection into developing Xenopus laevis embryos. This strategy involves flanking expression cassettes of interest with inverted terminal repeat sequences (ITRs) from adeno-associated virus. Our results suggest that the ITR strategy may be generally applicable to other systems, such as zebra fish and embryonic stem cells, and may enable tissue-specific expression of transgenes in problematic contexts.