Pattern and kinetics of mouse donor spermatogonial stem cell colonization in recipient testes
Pattern and kinetics of mouse donor spermatogonial stem cell colonization in recipient testes
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DOI：
10.1095/biolreprod60.6.1429
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发表时间：
1999-06-01
影响因子：
3.6
通讯作者：
Brinster, RL
中科院分区：
文献类型：
--
作者：
Nagano, M;Avarbock, MR;Brinster, RL
最近开发了一种系统，其中移植的供体精子干细胞在不育受体的睾丸中建立完整的精子发生。为了深入了解干细胞的活性和供体生殖细胞的行为，我们分析了移植后4个月小鼠精原细胞在受体精管中的定植模式和动力学。定植过程可分为三个连续的阶段。首先，在最初的一周内，移植细胞随机分布在小管中，少数细胞到达基底膜。第二，从1周到1个月，基底膜上的供体细胞分裂并形成单层网络。第三，从大约1月龄开始并持续到整个观察期间，网络中心的细胞广泛分化并建立了精子发生群体，并通过重复第二阶段和第三阶段向外侧扩张。将10(6)个细胞移植到受体睾丸的精管中，平均可产生19个供体细胞衍生集落；在1 ~ 4月龄期间，菌落位点数量没有变化，但菌落长度从0.73 mm增加到5.78 mm。这些实验为系统定量研究菌落过程的模式和动力学奠定了可行性。利用精原细胞移植作为一种功能测定，应该有可能评估各种治疗对干细胞和受体精管的影响，从而为精子发生过程提供独特的见解。
Recently a system was developed in which transplanted donor spermatogonial stem cells establish complete spermatogenesis in the testes of an infertile recipient. To obtain insight into stem cell activity and the behavior of donor germ cells, the pattern and kinetics of mouse spermatogonial colonization in recipient seminiferous tubules were analyzed during the 4 mo following transplantation. The colonization process can be divided into three continuous phases. First, during the initial week, transplanted cells were randomly distributed throughout the tubules, and a small number reached the basement membrane. Second, from 1 wk to 1 mo, donor cells on the basement membrane divided and formed a monolayer network. Third, beginning at about 1 mo and continuing throughout the observation period, cells in the center of the network differentiated extensively and established a colony of spermatogenesis, which expanded laterally by repeating phase two and then three. An average of 19 donor cell-derived colonies developed from 10(6) cells transplanted to the seminiferous tubules of a recipient testis; the number of colonized sites did not change between 1 and 4 mo. However, the length of the colonies increased from 0.73 to 5.78 mm between 1 and 4 mo. These experiments establish the feasibility of studying in a systematic and quantitative manner the pattern and kinetics of the colonization process. Using spermatogonial transplantation as a functional assay, it should be possible to assess the effects of various treatments on stem cells and on recipient seminiferous tubules to provide unique insight into the process of spermatogenesis.