Bacterial DNA activates immunity in Arabidopsis thaliana
Bacterial DNA activates immunity in Arabidopsis thaliana
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DOI：
10.1007/s10327-009-0162-4
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发表时间：
2009-04
影响因子：
1.2
通讯作者：
S. Yakushiji;Y. Ishiga;Y. Inagaki;K. Toyoda;T. Shiraishi;Y. Ichinose
中科院分区：
文献类型：
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作者：
S. Yakushiji;Y. Ishiga;Y. Inagaki;K. Toyoda;T. Shiraishi;Y. Ichinose
为了启动对病原生物入侵的防御反应，动物和植物必须识别微生物相关分子模式（MAMP）。本研究以模式植物拟南芥（Arabidopsis thaliana）为材料，研究了细菌DNA的诱导活性，结果表明，经EcoRI酶切的质粒DNA可诱导拟南芥产生活性氧和胼胝质沉积等防御反应，而经SmaI和HapII酶切的质粒DNA和经EcoRI酶切的鲱鱼DNA则不能显著诱导这些反应。此外，质粒DNA和大肠杆菌DNA的CpG序列的甲基化降低了防御反应的水平。内吞抑制剂渥曼青霉素和金刚烷胺显著抑制DNA诱导的防御反应。这些结果表明，非甲基化的CpG DNA，作为MAMP，诱导防御反应inhibitors和非甲基化的DNA似乎是通过内吞易位到细胞质中。
To initiate defense responses against invasion of pathogenic organisms, animals and plants must recognize microbe-associated molecular patterns (MAMPs). In this study, the elicitor activity of bacterial DNA on the model plantArabidopsis thalianawas examined.EcoRI-digested plasmid DNA induced defense responses such as generation of reactive oxygen species and deposition of callose, whereasSmaI- andHapII-digested plasmid DNA andEcoRI-digested herring DNA did not remarkably induce these responses. Further, methylation of the CpG sequence of plasmid DNA andEscherichia coliDNA reduced the level of the defense responses. The endocytosis inhibitors wortmannin and amantadine significantly inhibited DNA-induced defense responses. These results suggest that non-methylated CpG DNA, as a MAMP, induced defense responses inArabidopsisand that non-methylated DNA seems to be translocated into the cytoplasm by endocytosis.