Superoxide dismutase in Arabidopsis: An eclectic enzyme family with disparate regulation and protein localization
Superoxide dismutase in Arabidopsis: An eclectic enzyme family with disparate regulation and protein localization
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DOI：
10.1104/pp.118.2.637
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发表时间：
1998-10-01
期刊：
影响因子：
7.4
通讯作者：
Last, RL
中科院分区：
文献类型：
--
作者：
Kliebenstein, DJ;Monde, RA;Last, RL
许多环境胁迫会导致植物组织内超氧化物的产生增加，并且植物被认为依靠超氧化物歧化酶（SOD）来解毒这种活性氧。我们已经在拟南芥中鉴定了 7 个 SOD cDNA 和基因。它们由三种 CuZnSOD（CSD1、CSD2 和 CSD3）、三种 FeSOD（FSD1、FSD2 和 FSD3）和一种 MnSOD (MSD1) 组成。这七个 SOD 基因的染色体位置已经确定。为了研究该酶家族，生成了针对五种蛋白质的抗体：CSD1、CSD2、CSD3、FSD1 和 MSD1。使用这些抗血清和非变性聚丙烯酰胺凝胶电泳酶测定，我们鉴定了拟南芥莲座组织中两种 CuZnSOD 以及 FeSOD 和 MnSOD 的蛋白质和活性。此外，亚细胞分级分离研究揭示了拟南芥叶绿体中存在 CSD2 和 FeSOD 蛋白。七种 SOD mRNA 和四种蛋白质在不同光照条件、臭氧熏蒸和 UV-B 照射下受到差异性调节。据我们所知，这是第一份对植物物种中多种 SOD 蛋白的调控进行大规模分析的报告。
A number of environmental stresses can lead to enhanced production of superoxide within plant tissues, and plants are believed to rely on the enzyme superoxide dismutase (SOD) to detoxify this reactive oxygen species. We have identified seven cDNAs and genes for SOD in Arabidopsis. These consist of three CuZnSODs (CSD1, CSD2, and CSD3), three FeSODs (FSD1, FSD2, and FSD3), and one MnSOD (MSD1). The chromosomal location of these seven SOD genes has been established. To study this enzyme family, antibodies were generated against five proteins: CSD1, CSD2, CSD3, FSD1, and MSD1. Using these antisera and nondenaturing-polyacrylamide gel electrophoresis enzyme assays, we identified protein and activity for two CuZnSODs and for FeSOD and MnSOD in Arabidopsis rosette tissue. Additionally, subcellular fractionation studies revealed the presence of CSD2 and FeSOD protein within Arabidopsis chloroplasts. The seven SOD mRNAs and the four proteins identified were differentially regulated in response to various light regimes, ozone fumigation, and ultraviolet-B irradiation. To our knowledge, this is the first report of a large-scale analysis of the regulation of multiple SOD proteins in a plant species.