Rambutan-liked Pickering emulsion stabilized by cellulose nanocrystals for enhancing anti-bacterial activity and anti-inflammatory effect of Chimonanthus nitens Oliv. essential oil.
Rambutan-liked Pickering emulsion stabilized by cellulose nanocrystals for enhancing anti-bacterial activity and anti-inflammatory effect of Chimonanthus nitens Oliv. essential oil.
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DOI：
10.1016/j.ijbiomac.2023.124665
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发表时间：
2023-04
影响因子：
8.2
通讯作者：
Shuiyan Chen;Huazhang Lai;Xiaoyu Su;Huaping Yu;Biao Li;Shaofeng Wei;Mingming Yang;Yongping Wang;Baode Shen;Pengfei Yue
中科院分区：
文献类型：
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作者：
Shuiyan Chen;Huazhang Lai;Xiaoyu Su;Huaping Yu;Biao Li;Shaofeng Wei;Mingming Yang;Yongping Wang;Baode Shen;Pengfei Yue
由于蜡梅具有挥发性和水溶性差，其医药用途受到限制。精油（CEO）在医学领域的使用受到严格限制。为了解决这个问题，通过涂覆羧甲基纤维素钠改性纤维素纳米晶（CCN）作为稳定剂，有效地设计了一种具有尖峰表面的新型CEO负载红毛丹类皮克林乳液（CEO-RPE）。研究了 CCN 浓度对 CEO-RPE 形成和稳定的影响。结果表明，用 1% CCN 稳定的 CEO-RPE 具有较小的液滴尺寸，并表现出类似红毛丹的表面，并且由于 CCN 覆盖在液滴表面的空间屏障，在浓盐和高 pH 条件下保持稳定。随后，研究了CEO-RPE对大肠杆菌的抗菌性能。大肠杆菌，S。金黄色葡萄球菌，P.铜绿假单胞菌，和S.通过测定最低抑菌浓度 (MIC) 来抑制肺炎支原体。结果表明，与CEO相比，CEO-RPE表现出更高的抗菌活性，这可能归因于其对细菌细胞膜的有效粘附。此外，抗炎实验结果表明，CEO-RPE对脂多糖（LPS）诱导的大鼠急性肺损伤（ALI）也表现出较强的抗炎作用。因此，CCN 稳定的红毛丹样 Pickering 乳液似乎是提高 CEO 抗菌和抗炎活性的一种有前途的策略。
Owing to volatility and poor water solubility, the medical application ofChimonanthus nitens Oliv. essential oil (CEO)in the fields of medicine was strictly limited. To tackle this problem, a novel CEO loaded rambutan-liked Pickering emulsion (CEO-RPE) with a spiky surface was effectively designed by coating with carboxymethyl cellulose sodium modified cellulose nanocrystals (CCN) as stabilizer. The effect of CCN concentration on the formation and stabilization of CEO-RPE was investigated. The results showed that CEO-RPE stabilized by 1 % CCN had a smaller droplet size and exhibited a rambutan-liked surface, and was stabilized against concentrated salt and high pH condition due to the steric barrier of CCN that covered in the droplet surface. Subsequently, the antibacterial performance of CEO-RPE was investigated againstE. coli,S. aureus,P. aeruginosa, andS. pneumoniaeby determining the minimum inhibitory concentration (MIC). The results showed that the CEO-RPE exhibited higher antibacterial activity compared to CEO, which could be attributed to its effective adhesion to the cell membrane of bacteria. In addition, the results of anti-inflammatory experiments showed that CEO-RPE also exhibited strong anti-inflammatory effect on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. Therefore, the CCN stabilized rambutan-liked Pickering emulsion seemed to be a promising strategy to increase the antibacterial and anti-inflammatory activity of CEO.