Platinum-Decorated Gold Nanoparticles with Dual Functionalities for Ultrasensitive Colorimetric in Vitro Diagnostics
Platinum-Decorated Gold Nanoparticles with Dual Functionalities for Ultrasensitive Colorimetric in Vitro Diagnostics
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DOI：
10.1021/acs.nanolett.7b02385
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发表时间：
2017-09-01
期刊：
影响因子：
10.8
通讯作者：
Xia, Xiaohu
中科院分区：
文献类型：
--
作者：
Gao, Zhuangqiang;Ye, Haihang;Xia, Xiaohu
金纳米颗粒（AuNPs）作为信号报告者已经在体外比色诊断（IVDs）中使用了几十年。然而，由于受aunp固有等离子体动力学的限制，大幅度提高基于aunp的ivd的检测灵敏度仍然是一个巨大的挑战。在这项工作中，我们通过开发独特的双功能aunp来绕过这种限制，这种aunp是通过在传统的aunp上涂覆亚10原子层的超薄Pt皮肤（即Au@Pt NPs）来设计的。Au@Pt NPs保留了初始AuNPs的等离子体活性，同时具有Pt表皮赋予的超高催化活性。这样的双重功能，等离子体和催化，提供了两种不同的检测选择：一种是由等离子体产生的颜色（低灵敏度模式），另一种是由显色基质催化的更敏感的颜色（高灵敏度模式），实现了检测性能的“按需”调整。以横向流动试验为模型IVD平台，以传统的AuNPs为基准，我们证明Au@Pt NPs可以将检测灵敏度提高2个数量级。
Au nanoparticles (AuNPs) as signal reporters have been utilized in colorimetric in vitro diagnostics (IVDs) for decades. Nevertheless, it remains a grand challenge to substantially enhance the detection sensitivity of AuNP-based IVDs as confined by the inherent plasmonics of AuNPs. In this work, we circumvent this confinement by developing unique dual-functional AuNPs that were engineered by coating conventional AuNPs with ultrathin Pt skins of sub-10 atomic layers (i.e., Au@Pt NPs). The Au@Pt NPs retain the plasmonic activity of initial AuNPs while possessing ultrahigh catalytic activity enabled by Pt skins. Such dual functionalities, plasmonics and catalysis, offer two different detection alternatives: one produced just by the color from plasmonics (low-sensitivity mode) and the second more sensitive color catalyzed from chromogenic substrates (high-sensitivity mode), achieving an "on-demand" tuning of the detection performance. Using lateral flow assay as model IVD platform and conventional AuNPs as a benchmark, we demonstrate that the Au@Pt NPs could enhance detection sensitivity by 2 orders of magnitude.