Separation of amino acid homopolymers by capillary gel electrophoresis.
Separation of amino acid homopolymers by capillary gel electrophoresis.
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通过毛细管凝胶电泳分离氨基酸均聚物。
DOI：
10.1021/ac00053a012
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发表时间：
1993
影响因子：
7.4
通讯作者：
Novotny,MV
中科院分区：
文献类型：
--
作者：
Dolník,V;Novotny,MV
在毛细管电泳中，采用高浓度和适度交联的丙烯酰胺型凝胶填充毛细管，成功地用于分离各种聚氨基酸的寡聚体。阴离子和阳离子性质的混合物均得到充分解析。在220 nm处的紫外检测中，N端基团的聚阴离子也可以用3-（4-羧基苯甲酰基）-2-喹啉甲醛（CBQCA）标记，以提高激光诱导荧光检测的灵敏度。
Gel-filled capillaries utilizing highly concentrated and mod-erately cross-linked acrylamide-type gels In capillary electrophoresis were successfully applied to the separation of the Individual oligomers of various poly (amino acids). Mixtures of both anionic and cationic nature were adequately resolved. While UV detection at 220 nm was mostly utmzed, the polyanions with N-termlnal groupscan also be tagged with 3-(4-carboxybenzoyl)-2-qulnollnecarboxaldehyde (CBQCA) for a more sensitive detection by a laser-induced fluorescence detector.