The developmental genetics of hybrid inviability: a mitotic defect in Drosophila hybrids.
The developmental genetics of hybrid inviability: a mitotic defect in Drosophila hybrids.
复制标题
杂交不存活的发育遗传学：果蝇杂交中的有丝分裂缺陷。
DOI：
10.1093/genetics/145.4.1031
复制
发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Hawley,RS
中科院分区：
文献类型：
--
作者：
Orr,HA;Madden,LD;Coyne,JA;Goodwin,R;Hawley,RS
我们报道了在果蝇黑腹复合体中杂交丧失活力的发育基础的研究。这些杂交种的病理与有丝分裂突变体Ind非常相似。黑猩猩。我们使用马赛克和细胞学分析表明，杂交种雄性不存活与有丝分裂细胞分裂缺陷有关，而且可能是由该缺陷引起的。在马赛克研究中，我们发现，在其他雌性杂交中产生的雄性克隆不是对细胞致命的，但非常小，可能反映了有丝分裂增殖的缺陷。对幼虫神经母细胞的细胞学检查显示，杂交后代中存在严重的有丝分裂缺陷：即使在秋水仙素中孵育2小时后，染色体也几乎完全不能凝聚。克隆增殖和染色质浓缩的缺陷都可以通过已知的突变来挽救通常无法存活的杂交雄性。我们提出了一个简单的模型，在这个模型中，杂交不能部分或全部由有丝分裂缺陷引起；这种缺陷反过来又是由D的杂交男性救援(HMR)基因座之间的相互作用引起的。黑素基因和常染色体基因(S)。黑猩猩的姊妹物种。
We report studies of the developmental basis of hybrid inviability in theDrosophila melanogastercomplex. The pathology of these hybrids closely resembles that of mitotic mutants inD. melanogaster. We use mosaic and cytological analyses to show that hybrid male inviability is associated with, and probably caused by, a defect in mitotic cell division. In the mosaic study, we find that male clones produced in otherwise female hybrids are not cell lethal but are very small, probably reflecting defects in mitotic proliferation. Cytological inspection of larval neuroblasts reveals a profound mitotic defect in hybrids: chromosomes show a near-complete failure to condense even after 2 hr of incubation in colchicine. Both the defect in clonal proliferation and in chromatin condensation are rescued by mutations known to rescue normally inviable hybrid males. We present a simple model in which hybrid inviability is partly or entirely caused by a mitotic defect; this defect is, in turn, caused by an interaction between theHybrid male rescue(Hmr) locus ofD. melanogasterand autosomal gene(s) fromD. melanogaster's sister species.