Uncoupling apical constriction from tissue invagination
Uncoupling apical constriction from tissue invagination
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DOI：
10.7554/elife.22235.001
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发表时间：
2017-03-06
期刊：
影响因子：
7.7
通讯作者：
Andrew, Deborah J.
中科院分区：
文献类型：
--
作者：
Chung, Se Yeon;Kim, Sangjoon;Andrew, Deborah J.
根尖缩窄是一种广泛应用的细胞形状变化，与极化上皮的折叠、弯曲和内陷有关。目前尚不清楚在组织内陷过程中，根尖收缩是如何在时空上受到调节的，以及这个细胞过程如何在不同的发育背景下促进管的形成。以果蝇唾液腺（SG）内陷为模型，我们发现叉头转录因子对折叠原肠胚表达的调控是协调顶端收缩的Rho激酶和非肌球蛋白II的顶端内侧积累所必需的。我们证明，尽管这样的操作会影响内陷的几何形状，但空间协调的根尖收缩的丧失和根尖收缩的完全阻塞都不会阻止内化和管的形成。当根尖收缩被破坏时，组织水平肌球蛋白索产生的压缩力有助于SG内陷。我们证明了完全伸长的极化SGs可以在胚胎外形成，这表明管的形成和伸长是SG的固有特性。
Apical constriction is a widely utilized cell shape change linked to folding, bending and invagination of polarized epithelia. It remains unclear how apical constriction is regulated spatiotemporally during tissue invagination and how this cellular process contributes to tube formation in different developmental contexts. Using Drosophila salivary gland (SG) invagination as a model, we show that regulation of folded gastrulation expression by the Fork head transcription factor is required for apicomedial accumulation of Rho kinase and non-muscle myosin II, which coordinate apical constriction. We demonstrate that neither loss of spatially coordinated apical constriction nor its complete blockage prevent internalization and tube formation, although such manipulations affect the geometry of invagination. When apical constriction is disrupted, compressing force generated by a tissue-level myosin cable contributes to SG invagination. We demonstrate that fully elongated polarized SGs can form outside the embryo, suggesting that tube formation and elongation are intrinsic properties of the SG.