Plant phenylacetaldehyde synthase is a bifunctional homotetrameric enzyme that catalyzes phenylalanine decarboxylation and oxidation
Plant phenylacetaldehyde synthase is a bifunctional homotetrameric enzyme that catalyzes phenylalanine decarboxylation and oxidation
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DOI：
10.1074/jbc.m602708200
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发表时间：
2006-08-18
影响因子：
4.8
通讯作者：
Dudareva, Natalia
中科院分区：
文献类型：
--
作者：
Kaminaga, Yasuhisa;Schnepp, Jennifer;Dudareva, Natalia
我们分离并鉴定了矮牵牛品种。米切尔苯乙醛合酶 (PAAS)，催化苯乙醛（花香成分）的形成。 PAAS 是一种胞质同源四聚酶，属于 II 类吡哆醛 5'-磷酸依赖性氨基酸脱羧酶，与植物 L-酪氨酸/3,4-二羟基-L-苯丙氨酸和 L-色氨酸脱羧酶具有广泛的氨基酸一致性（接近 65%）。它对苯丙氨酸表现出严格的特异性，表观 Km 为 1.2 mM。 PAAS 是一种双功能酶，可催化苯丙氨酸脱羧与氧化之间前所未有的高效偶联，产生化学计量的苯乙醛、CO2、氨和过氧化氢。
We have isolated and characterized Petunia hybrida cv. Mitchell phenylacetaldehyde synthase (PAAS), which catalyzes the formation of phenylacetaldehyde, a constituent of floral scent. PAAS is a cytosolic homotetrameric enzyme that belongs to group II pyridoxal 5'-phosphate-dependent amino-acid decarboxylases and shares extensive amino acid identity (similar to 65%) with plant L-tyrosine/ 3,4-dihydroxy-L-phenylalanine and L-tryptophan decarboxylases. It displays a strict specificity for phenylalanine with an apparent Km of 1.2 mM. PAAS is a bifunctional enzyme that catalyzes the unprecedented efficient coupling of phenylalanine decarboxylation to oxidation, generating phenylacetaldehyde, CO2, ammonia, and hydrogen peroxide in stoichiometric amounts.