Molecular characterization and expression profile of methionine sulfoxide reductase gene family in maize (Zea mays) under abiotic stresses.
Molecular characterization and expression profile of methionine sulfoxide reductase gene family in maize (Zea mays) under abiotic stresses.
复制标题
DOI：
10.1016/j.gene.2015.02.066
复制
发表时间：
2015-05
期刊：
影响因子：
3.5
通讯作者：
Jiantang Zhu;Pengcheng Ding;Qingqing Li;Yankun Gao;Fanguo Chen;G. Xia
中科院分区：
文献类型：
--
作者：
Jiantang Zhu;Pengcheng Ding;Qingqing Li;Yankun Gao;Fanguo Chen;G. Xia
甲硫氨酸(Met)氧化为甲硫氨酸亚砜(Metso)是一种常见的损伤形式，是由各种环境胁迫引起的活性氧(ROS)积累造成的。蛋氨酸亚砜还原酶(MSR)修复氧化的蛋氨酸，保护生物体免受氧化损伤。根据底物的立体特异性，已经鉴定出两种类型的MSR，A和B，它们没有序列相似性。在本研究中，我们从两个公共数据库中鉴定了编码推定的MSR的六个基因。我们将它们与6个物种的MSR进行了比较，并对它们的分子特征、系统发育分析、三级结构和保守基序进行了评估。根据电子显微镜和定量逆转录聚合酶链式反应的实验数据，我们分析了ZmMSR基因在玉米不同器官中的cDNA序列和表达模式。我们发现，ZmMSR基因是由聚乙二醇和氯化钠诱导的，这两种物质都能产生氧化应激。结果表明，MSR在不同物种中是保守的，这表明不同物种之间的MSR具有与不同防御反应相关的共同机制。
Methionine (Met) oxidation to methionine sulfoxide (MetSO) is a common form of damage caused by reactive oxygen species (ROS) accumulation via various environmental stresses. Methionine sulfoxide reductase (MSR) repairs oxidized Met and protects organisms from oxidative damage. Two types of MSR, A and B, have been identified based on substrate stereo specificity; they share no sequence similarity. In the present study, we characterized six genes encoding the putative MSR from two public databases. We compared them with MSRs from 6 species, and evaluated molecular characterization, phylogenetic analysis, tertiary structure and conserved motifs. On the basis of in silico and the qRT-PCR experimental data, we analyzed cDNA sequences and expression patterns ofZmMSRgenes in different organs in maize. We found thatZmMSRgenes were induced by polyethylene glycol (PEG) and NaCl, both known to generate oxidative stress. The results show that MSRs are conserved in different species, suggesting that MSRs across different species share common mechanisms related to diverse defense responses.