ENHANCED PRESERVATION OF ORTHOTOPICALLY TRANSPLANTED RAT LUNGS BY NITROGLYCERIN BUT NOT HYDRALAZINE - REQUIREMENT FOR GRAFT VASCULAR HOMEOSTASIS BEYOND HARVEST VASODILATION
ENHANCED PRESERVATION OF ORTHOTOPICALLY TRANSPLANTED RAT LUNGS BY NITROGLYCERIN BUT NOT HYDRALAZINE - REQUIREMENT FOR GRAFT VASCULAR HOMEOSTASIS BEYOND HARVEST VASODILATION
复制标题
DOI：
10.1161/01.res.76.5.900
复制
发表时间：
1995-05-01
影响因子：
20.1
通讯作者：
PINSKY, DJ
中科院分区：
文献类型：
--
作者：
NAKA, Y;CHOWDHURY, NC;PINSKY, DJ
肺内产生的一氧化氮（NO）维持肺血管稳态特性，调节白细胞运输、血小板聚集和血管紧张素。由于在再灌注过程中产生的活性氧中间产物与可用的NO迅速反应，我们假设NO供体硝酸甘油（NTG）将通过改善移植物血流和减少移植物中性粒细胞和血小板隔离来增强移植的肺保存。通过使用原位大鼠左肺移植模型，结扎天然右肺动脉以确保受体存活和生理测量完全取决于移植的肺，在单独的Euro-Collins溶液（EC）或EC与补充NTG中保存6小时4 ℃后，在70只雄性刘易斯大鼠中进行移植。与单独EC相比，补充NTG显著增加肺动脉流量（2.2+/-1.4至21.4+/-2.9 mL/min，P
Nitric oxide (NO) produced within the lungs maintains pulmonary vascular homeostatic properties, modulating leukocyte traffic, platelet aggregation, and vasomotor tone. Because reactive oxygen intermediates generated during reperfusion react rapidly with available NO, we hypothesized that the NO donor nitroglycerin (NTG) would enhance lung preservation for transplantation by improving graft blood flow and reducing graft neutrophil and platelet sequestration. By use of an orthotopic rat left lung transplant model, with ligation of the native right pulmonary artery to ensure that recipient survival and physiological measurements depend entirely on the transplanted lung, transplants were performed in 70 male Lewis rats after 6-hour 4 degrees C preservation in Euro-Collins solution (EC) alone or EC with supplemental NTG. Compared with EC alone, supplemental NTG significantly increased pulmonary arterial flow (2.2+/-1.4 to 21.4+/-2.9 mL/min, P