Axonal outgrowth of hippocampal neurons on micro-scale networks of polylysine-conjugated laminin
Axonal outgrowth of hippocampal neurons on micro-scale networks of polylysine-conjugated laminin
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DOI：
10.1016/s0142-9612(00)00352-5
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发表时间：
2001-05-01
期刊：
影响因子：
14
通讯作者：
Bizios, R
中科院分区：
文献类型：
--
作者：
Kam, L;Shain, W;Bizios, R
微接触印刷用于定义聚赖氨酸缀合层粘连蛋白的互连晶格网络，层粘连蛋白是一种蛋白质-多肽连接物，是材料表面神经元生长的有效促进剂。在血清蛋白存在的情况下，大鼠海马神经元选择性地粘附到宽度窄至 2.6 微米的聚赖氨酸缀合的层粘连蛋白的特征上。粘附神经元延长了长轴突，其精确地遵循且不偏离规定的模式，这表明神经元对这种蛋白质具有高选择性的反应，并且这些技术有效地提供了轴突生长的远程指导。在无血清细胞培养条件下对神经元反应的进一步检查表明，聚赖氨酸缀合的层粘连蛋白的生长促进活性归因于具有生物活性的层粘连蛋白。总之，这些结果表明，聚赖氨酸缀合的层粘连蛋白以不依赖于血清的方式在材料表面上提供神经元附着和轴突生长的高精度引导。这种在低密度、无血清培养中高精度引导海马神经元反应的能力对于开发先进的基于神经元的设备非常有价值。 (C) 2001 Elsevier Science Ltd. 保留所有权利。
Microcontact printing was used to define an interconnected lattice network of polylysine-conjugated laminin, a protein-polypeptide ligate that is an effective promoter of neuron outgrowth on material surfaces. In the presence of serum proteins, rat hippocampal neurons selectively adhered to features of polylysine-conjugated laminin as narrow as 2.6 mum in width. Adhering neurons extended long axonal processes, which precisely followed and did not deviate from the prescribed patterns, demonstrating that neurons respond to this protein with high selectivity and that these techniques effectively provide long-range guidance of axonal outgrowth. Further examination of neuron response under serum-free cell culture conditions demonstrated that the outgrowth-promoting activity of polylysine-conjugated laminin was attributed to biologically active laminin. Together, these results demonstrate that polylysine-conjugated laminin provides for high-precision guidance of neuron attachment and axon outgrowth on material surfaces in a serum-independent manner. This ability to guide hippocampal neuron response in low-density, serum-free culture with high precision is valuable for the development of advanced, neuron-based devices. (C) 2001 Elsevier Science Ltd. All rights reserved.