Simultaneous analysis of mitragynine, 7-hydroxymitragynine, and other alkaloids in the psychotropic plant "kratom" (Mitragyna speciosa) by LC-ESI-MS
Simultaneous analysis of mitragynine, 7-hydroxymitragynine, and other alkaloids in the psychotropic plant "kratom" (Mitragyna speciosa) by LC-ESI-MS
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DOI：
10.1007/s11419-009-0070-5
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发表时间：
2009-07-01
影响因子：
2.2
通讯作者：
Goda, Yukihiro
中科院分区：
文献类型：
--
作者：
Kikura-Hanajiri, Ruri;Kawamura, Maiko;Goda, Yukihiro
帽柱木（Mitragyna spiosa）是一种被称为“kratom”的热带植物，其叶子传统上在泰国和马来西亚被用作鸦片的替代品。帽柱木碱是大花帽柱木的主要成分，具有阿片类药物激动活性，其衍生物 7-羟基帽柱木碱 (7-OH-帽柱木碱)（次要成分）比帽柱木碱或吗啡更有效。最近，许多含有这种植物的产品因其预期的麻醉作用而在日本药品市场上以“香”的形式流通。尽管帽柱木碱和 7-OH-帽柱木碱具有效力且滥用分布广泛，但目前尚无卡痛叶原料或商业产品中帽柱木碱和 7-OH-帽柱木碱的定量分析报告。本研究采用液相色谱-电喷雾电离质谱 (LC-ESI-MS) 开发了一种同时分析卡痛原料和商业产品中帽柱木碱、7-OH-帽柱木碱和其他吲哚生物碱（奇木木碱、特奇西里汀和派南替因）的方法。通过该方法，在13个产品中的11个产品中检测到帽柱木碱、7-OH-帽柱木碱和其他生物碱。产品中帽柱木碱含量为1%~6%，7-OH-帽柱木碱含量为0.01%~0.04%。由于 7-OH-帽柱木碱的效力比吗啡强得多，因此大花帽柱木的滥用是一个值得关注的问题。本分析方法被认为可用于筛选药品市场上的 M. spiosa 产品。
The leaves of Mitragyna speciosa, a tropical plant known as "kratom," have been traditionally used as a substitute for opium in Thailand and Malaysia. Mitragynine, a major constituent of M. speciosa, has an opioid agonistic activity, and its derivative 7-hydroxymitragynine (7-OH-mitragynine) (a minor constituent) is much more potent than mitragynine or morphine. Recently, many products containing this plant have been distributed as "incense" on the drug market in Japan for their expected narcotic effects. Despite their potency and their wide distribution for abuse, there are no reports on the quantitative analysis of mitragynine and 7-OH-mitragynine in the raw materials or in the commercial products of kratom. In this study, a method for simultaneous analysis of mitragynine, 7-OH-mitragynine, and other indole alkaloids (speciogynine, speciociliatine, and paynantheine), present in the raw materials and commercial products of kratom, was developed using liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS). By this method, mitragynine, 7-OH-mitragynine, and the other alkaloids were detected in 11 of the 13 products. The content of mitragynine in the products ranged from 1% to 6%, and that of 7-OH-mitragynine from 0.01% to 0.04%. Because 7-OH-mitragynine is much more potent than morphine, M. speciosa abuse is a matter of major concern. The present analytical method is considered useful for the screening of M. speciosa products in the drug market.