Coastal marine habitats harbor novel early-diverging fungal diversity
Coastal marine habitats harbor novel early-diverging fungal diversity
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DOI：
10.1016/j.funeco.2016.10.006
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发表时间：
2017-02-01
期刊：
影响因子：
2.9
通讯作者：
Picard, Kathryn T.
中科院分区：
文献类型：
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作者：
Picard, Kathryn T.
尽管经过近一个世纪的研究，海洋真菌的多样性仍然知之甚少。利用显微镜或依赖培养的方法进行的历史调查表明，海洋真菌的种类相对较少，主要是Dikarya，并且局限于沿海栖息地。然而，利用高通量测序技术来表征微生物群落，通过揭示来自陆地和水生栖息地的新型种型，挑战了真菌多样性的传统概念。在这里，我使用核糖体大亚基（LSU/28S）的离子半导体测序（ion Torrent）来探索从北卡罗莱纳沿海四个栖息地收集的水和沉积物样品中的真菌多样性。虽然所有真菌门都有代表，但主要分类群为子囊菌门和壶菌门。浅滩和湿地沉积物的多样性最高，底栖沉积物的新序列比例最高。大多数划分为早期分化真菌类群的序列在统计支持下无法划分出门，这表明它们属于未知的谱系。(C) 2016 Elsevier Ltd和British myologicalsociety。版权所有。
Despite nearly a century of study, the diversity of marine fungi remains poorly understood. Historical surveys utilizing microscopy or culture-dependent methods suggest that marine fungi are relatively species-poor, predominantly Dikarya, and localized to coastal habitats. However, the use of high throughput sequencing technologies to characterize microbial communities has challenged traditional concepts of fungal diversity by revealing novel phylotypes from both terrestrial and aquatic habitats. Here, I used ion semiconductor sequencing (Ion Torrent) of the ribosomal large subunit (LSU/28S) to explore fungal diversity from water and sediment samples collected from four habitats in coastal North Carolina. The dominant taxa observed were Ascomycota and Chytridiomycota, though all fungal phyla were represented. Diversity was highest in sand flats and wetland sediments, though benthic sediments harbored the highest proportion of novel sequences. Most sequences assigned to early-diverging fungal groups could not be assigned beyond phylum with statistical support, suggesting they belong to unknown lineages. (C) 2016 Elsevier Ltd and British Mycological Society. All rights reserved.