Activated signal transduction kinases frequently occupy target genes
Activated signal transduction kinases frequently occupy target genes
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DOI：
10.1126/science.1127677
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发表时间：
2006-07-28
期刊：
影响因子：
56.9
通讯作者：
Young, Richard A.
中科院分区：
文献类型：
--
作者：
Pokholok, Dmitry K.;Zeitlinger, Julia;Young, Richard A.
细胞信号转导通路改变基因表达程序以响应环境的变化，但是这些通路调节其控制下的基因群体的机制还不完全清楚。我们目前的证据表明，大多数丝裂原活化蛋白激酶和蛋白激酶A亚基成为物理相关的基因，他们在酵母（酿酒酵母）基因组中的调节。检测信号激酶与靶基因的这种相互作用的能力可以用于更精确和全面地绘制真核细胞用于响应其环境的调节电路。
Cellular signal transduction pathways modify gene expression programs in response to changes in the environment, but the mechanisms by which these pathways regulate populations of genes under their control are not entirely understood. We present evidence that most mitogen-activated protein kinases and protein kinase A subunits become physically associated with the genes that they regulate in the yeast ( Saccharomyces cerevisiae) genome. The ability to detect this interaction of signaling kinases with target genes can be used to more precisely and comprehensively map the regulatory circuitry that eukaryotic cells use to respond to their environment.