Oleic acid-loaded nanostructured lipid carrier inhibit neutrophil activities in the presence of albumin and alleviates skin inflammation
Oleic acid-loaded nanostructured lipid carrier inhibit neutrophil activities in the presence of albumin and alleviates skin inflammation
复制标题
DOI：
10.2147/ijn.s208489
复制
发表时间：
2019-01-01
影响因子：
8
通讯作者：
Hwang, Tsong-Long
中科院分区：
文献类型：
--
作者：
Chen, Chun-Yu;Lee, Ying-Hsuan;Hwang, Tsong-Long
目的：研究油酸（OA）在纳米结构脂质载体（OA- nlc）中的掺入，以改善白蛋白存在下的抗炎作用。材料和方法：通过热高剪切均质/超声法制备NLCs，对其粒径、zeta电位和毒性进行了表征。我们检测了OA-NLC对中性粒细胞活性的影响。利用白三烯b4诱导的小鼠皮肤炎症模型也阐明了皮肤治疗潜力。结果：在白蛋白存在的情况下，OA- nlc抑制超氧化物的生成和弹性蛋白酶的释放，而非游离OA。局部给予OA-NLC可减轻中性粒细胞浸润，减轻皮肤炎症的严重程度。结论：在NLC中掺入OA可以克服白蛋白的干扰，从而削弱OA的抗炎作用。OA-NLC在局部软膏中具有潜在的治疗作用。
Aim: This paper reports on the incorporation of oleic acid (OA) within nanostructured lipid carriers (OA-NLC) to improve the anti-inflammatory effects in the presence of albumin.Materials and methods: NLCs produced via hot high-shear homogenization/ultrasonication were characterized in terms of particle size, zeta potential, and toxicity. We examined the effects of OA-NLC on neutrophil activities. Dermatologic therapeutic potential was also elucidated by using a murine model of leukotriene B4-induced skin inflammation.Results: In the presence of albumin, OA-NLC but not free OA inhibited superoxide generation and elastase release. Topical administration of OA-NLC alleviated neutrophil infiltration and severity of skin inflammation.Conclusion: OA incorporated within NLC can overcome the interference of albumin, which would undermine the anti-inflammatory effects of OA. OA-NLC has potential therapeutic effects in topical ointments.