Complex induction of bovine uterine proteins by interferon-tau.
Complex induction of bovine uterine proteins by interferon-tau.
复制标题
干扰素-tau 复合诱导牛子宫蛋白。
DOI：
10.1095/biolreprod59.2.293
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发表时间：
1998
影响因子：
3.6
通讯作者：
Hansen,TR
中科院分区：
文献类型：
--
作者：
Staggs,KL;Austin,KJ;Johnson,GA;Teixeira,MG;Talbott,CT;Dooley,VA;Hansen,TR
干扰素-τ（IFN-τ）由孕体释放，并在妊娠早期诱导两种子宫蛋白：泛素交叉反应蛋白（UCRP）和粒细胞趋化蛋白-2（GCP-2）。本实验旨在确定胞质UCRP的检测（Western印迹）和GCP-2的释放是否可用于检查培养的子宫内膜外植体和原代上皮细胞中的IFN-τ信号转导。在外植体培养物中，重组（r）1型IFN（rboIFN-τ和rboIFN-α; 5、25、100 nM）诱导UCRP，但仅rboIFN-τ诱导GCP-2。在培养的原代上皮细胞中，含有天然IFN-τ的重组boIFN-τ和孕体分泌蛋白诱导UCRP和GCP-2。所有浓度的rboIFN-α（25，50，100 nM）诱导UCRP，但只有最高浓度诱导培养的原代上皮细胞中的GCP-2。有趣的是，佛波酯（100，500，1000 ng/ml）诱导GCP-2，但它对UCRP没有影响。由于1型IFN诱导UCRP，IFN-τ可能与Janus激酶（Jak）相关的IFN-α受体相互作用，磷酸化信号转导和转录激活因子（STAT）和/或干扰素调节因子-1（IRF-1）。然而，IFN-τ对GCP-2的特异性诱导可能涉及与蛋白激酶C和Jak/STAT/IRF-1途径相关的1型受体亚基或转录激活因子的变体。
Interferon-tau (IFN-τ) is released by the conceptus and induces two uterine proteins during early pregnancy: ubiquitin cross-reactive protein (UCRP) and granulocyte chemotactic protein-2 (GCP-2). The present experiments were designed to determine whether detection (Western blot) of cytosolic UCRP and release of GCP-2 could be used to examine IFN-τ signal transduction in cultured endometrial explants and primary epithelial cells. Recombinant (r) type 1 IFNs (rboIFN-τ and rboIFN-α; 5, 25, 100 nM) induced UCRP, but only rboIFN-τ induced GCP-2 in explant culture. Recombinant boIFN-τ and conceptus secretory proteins containing native IFN-τ induced UCRP and GCP-2 in cultured primary epithelial cells. All concentrations of rboIFN-α (25, 50, 100 nM) induced UCRP, but only the highest concentration induced GCP-2 in cultured primary epithelial cells. Interestingly, phorbol ester (100, 500, 1000 ng/ml) induced GCP-2, but it had no effect on UCRP. Because type 1 IFNs induce UCRP, IFN-τ probably interacts with the janus kinase (Jak)-associated IFN-α receptor to phosphorylate signal transducers and activators of transcription (STAT) and/or interferon regulatory factor-1 (IRF-1). However, IFN-τ-specific induction of GCP-2 may involve a variant type 1 receptor subunit or activators of transcription that are associated with protein kinase C and the Jak/STAT/IRF-1 pathway.