Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A.
Synthesis and characterization of water-soluble conjugated glycopolymer for fluorescent sensing of concanavalin A.
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DOI：
10.1002/asia.200900297
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发表时间：
2010-02
期刊：
影响因子：
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通讯作者：
Jianbing Shi;Liping Cai;Kan‐Yi Pu;B. Liu
中科院分区：
文献类型：
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作者：
Jianbing Shi;Liping Cai;Kan‐Yi Pu;B. Liu
采用Suzuki偶联聚合法合成了含20 mol% 2，1，3-苯并噻二唑（BT）的中性聚芴衍生物。随后通过不同的后聚合方法得到阳离子共轭聚合物A和带有α-甘露糖的聚合物B。由于带电荷的侧基或含糖基团连接到聚合物侧链上，A和B都显示出良好的水溶性。将伴刀豆球蛋白A（Con A）滴定到聚合物水溶液中导致聚合物A和B的不同荧光响应。聚合物A在与Con A相互作用时没有显示任何明显的荧光变化，而聚合物B在加入Con A时显示BT发射强度的荧光增加。这是因为α-甘露糖和Con A之间的特异性相互作用，其诱导聚合物聚集，然后促进从苯-芴片段到BT单元的能量转移。通过将BT发射强度的变化与Con A浓度相关联，获得了范围为1 nM至250 nM的实用校准曲线。基于聚合物B的Con A大分子探针的优点是它在Con A识别时显示出信号增加，这与其他基于缀合聚合物的荧光猝灭测定法显著不同。
A neutral polyfluorene derivative that contains 20 mol% 2,1,3-benzothiadiazole (BT) is synthesized by Suzuki cross-coupling polymerization. A cationic conjugated polymer A and an alpha-mannose-bearing polymer B are subsequently obtained through different post-polymerization methods. As a result of the charged pendant groups or sugar-bearing groups attached to the polymer side chains, both A and B show good water-solubility. The titration of Concanavalin A (Con A) into polymer aqueous solution leads to different fluorescent responses for polymers A and B. Polymer A does not show any obvious fluorescence change upon interaction with Con A, whereas polymer B shows fluorescence increase in BT emission intensity when Con A is added. This is because of the specific interaction between alpha-mannose and Con A, which induces polymer aggregation, and then facilitates energy transfer from the phenylene-fluorene segments to the BT units. A practical calibration curve ranging from 1 nM to 250 nM is obtained by correlating the changes in BT emission intensity with Con A concentration. The advantage of polymer B-based Con A macromolecular probe is that it shows signal increase upon Con A recognition, which is significantly different from other conjugated polymer-based fluorescence quenching assays.