Products of sulphide oxidation in extracts of Thiobacillus concretivorus.
Products of sulphide oxidation in extracts of Thiobacillus concretivorus.
复制标题
DOI：
10.1016/0005-2728(70)90025-3
复制
发表时间：
1970-03
期刊：
影响因子：
--
通讯作者：
D. Moriarty;D. Nicholas
中科院分区：
文献类型：
--
作者：
D. Moriarty;D. Nicholas
1.1.在无细胞提取物ofThiobacillusconcretivorus硫化物氧化的早期中间体是绑定到一个膜级分。该化合物可能处于元素硫的氧化水平，其性质表明它是线性聚合物形式，而不是更稳定的S8环结构。透析提取物将硫化物以及结合的硫中间体完全氧化为硫酸盐，而不形成硫代硫酸盐或连多硫酸盐。然而，在未透析的制剂中，无机硫化合物积累，硫化物形成的硫酸盐相当少。提出了硫化物通过结合硫聚合物和亚硫酸盐的酶氧化为硫酸盐的机理。
1.1. An early intermediate of sulphide oxidation in cell-free extracts ofThiobacillus concretivorusis bound to a membrane fraction.2.2. The compound is probably at the oxidation level of elemental sulphur, and its properties suggest that it is in a linear polymeric form rather than the more stable S8ring structure.3.3. Dialysed extracts oxidize sulphide as well as the bound sulphur intermediate completely to sulphate, without forming thiosulphate or polythionates. In undialysed preparations however inorganic sulphur compounds accumulate, and sulphate formation from sulphide is considerably less.4.4. A mechanism is proposed for the enzymic oxidation of sulphide to sulphateviaa bound sulphur polymer and sulphite.