Conversion of midbodies into germ cell intercellular bridges
Conversion of midbodies into germ cell intercellular bridges
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DOI：
10.1016/j.ydbio.2007.02.025
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发表时间：
2007-05-15
影响因子：
2.7
通讯作者：
Matzuk, Martin M.
中科院分区：
文献类型：
--
作者：
Greenbaum, Michael P.;Ma, Lang;Matzuk, Martin M.
而体细胞胞质分裂解决与分裂的中间体，导致独立的子细胞，生殖细胞胞质分裂的结论与形成一个稳定的细胞间桥互连的子细胞在合胞体。虽然已经发现了许多蛋白质的必需品，但直到最近，还没有发现哺乳动物生殖细胞细胞间桥形成所必需的蛋白质。使用TEX14作为生殖细胞间桥的标记，我们发现TEX14与centralspindlin复合物、有丝分裂驱动蛋白样蛋白1（MKLP 1）和雄性生殖细胞Rac GTP酶激活蛋白（MgcRacGAP）共定位，并将这些中间体基质蛋白转化为稳定的细胞间桥组分。相比之下，septins（SEPT）2、7和9是新形成的桥中的过渡蛋白。在培养的体细胞中，在没有其他生殖细胞特异性因子的情况下，TEX14可以定位于中间体，这表明TEX14起到将体细胞胞质分裂机制与其他生殖细胞蛋白质桥接的作用，以形成对雄性生殖至关重要的稳定的细胞间桥。由爱思唯尔公司出版
Whereas somatic cell cytokinesis resolves with abscission of the midbody, resulting in independent daughter cells, germ cell cytokinesis concludes with the formation of a stable intercellular bridge interconnecting daughter cells in a syncytium. While many proteins essential for abscission have been discovered, until recently, no proteins essential for mammalian germ cell intercellular bridge formation have been identified. Using TEX14 as a marker for the germ cell intercellular bridge, we show that TEX14 co-localizes with the centralspindlin complex, mitotic kinesin-like protein 1 (MKLP1) and male germ cell Rac GTPase-activating protein (MgcRacGAP) and converts these midbody matrix proteins into stable intercellular bridge components. In contrast, septins (SEPT) 2, 7 and 9 are transitional proteins in the newly forming bridge. In cultured somatic cells, TEX14 can localize to the midbody in the absence of other germ cell-specific factors, suggesting that TEX14 serves to bridge the somatic cytokinesis machinery to other germ cell proteins to form a stable intercellular bridge essential for male reproduction. Published by Elsevier Inc.