OXIDATION OF H-2, ORGANIC-COMPOUNDS AND INORGANIC SULFUR-COMPOUNDS COUPLED TO REDUCTION OF O-2 OR NITRATE BY SULFATE-REDUCING BACTERIA
OXIDATION OF H-2, ORGANIC-COMPOUNDS AND INORGANIC SULFUR-COMPOUNDS COUPLED TO REDUCTION OF O-2 OR NITRATE BY SULFATE-REDUCING BACTERIA
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DOI：
10.1007/bf00245211
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发表时间：
1992-07-01
影响因子：
2.8
通讯作者：
CYPIONKA, H
中科院分区：
文献类型：
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作者：
DANNENBERG, S;KRODER, M;CYPIONKA, H
所有14个测试的硫酸盐还原菌都能够用以下电子供体中的至少一种进行有氧呼吸：H-2、乳酸盐、丙酮酸盐、甲酸盐、乙酸盐、丁酸盐、乙醇、硫化物、硫代硫酸盐、亚硫酸盐。一般来说，我们没有获得以O2作为电子受体的生长。细菌是微需氧的，因为呼吸速率随着O2浓度的降低而增加，或者在重复O2添加后停止。消耗的O2的量表明，有机底物不完全氧化成乙酸盐;只有脱硫醇postgatei氧化乙酸盐与O2完全CO2。 许多菌株氧化亚硫酸盐（完全为硫酸盐）或硫化物（不完全，除Desulfobulbus propionicus）;硫代硫酸盐仅由脱硫脱硫弧菌菌株氧化;连三硫酸盐和连四硫酸盐不被任何菌株氧化。用脱硫脱硫弧菌CSN和丙酸脱硫菌对无机硫化物的氧化进行了详细的表征。D.脱硫剂在由多硫化物制备的亚硫酸盐、硫代硫酸盐或元素硫的氧化过程中形成硫酸盐。D.丙酸杆菌将亚硫酸盐和硫化物氧化成硫酸盐，元素硫主要氧化成硫代硫酸盐。发现了一条新的硫氮循环耦合途径：D.脱硫菌和（仅与亚硝酸盐）D.丙酸杆菌能够完全氧化硫化物，并将硝酸盐或亚硝酸盐还原为氨。两种菌株的无细胞提取物不氧化硫化物或硫代硫酸盐，但在亚硫酸盐氧化过程中形成ATP（37 nmol/100 nmol亚硫酸盐）。这一点以及AMP、焦磷酸盐和ATP对亚硫酸盐氧化的影响表明，硫酸盐是通过（反向）硫酸盐活化途径（涉及APS还原酶和ATP硫酸化酶）形成的。硫代硫酸盐氧化与O2可能需要一个还原的第一步，因为它是只获得与通电完整的细胞。
All of fourteen sulfate-reducing bacteria tested were able to carry out aerobic respiration with at least one of the following electron donors: H-2, lactate, pyruvate, formate, acetate, butyrate, ethanol, sulfide, thiosulfate, sulfite. Generally, we did not obtain growth with O2 as electron acceptor. The bacteria were microaerophilic, since the respiration rates increased with decreasing O2 concentrations or ceased after repeated O2 additions. The amounts of O2 consumed indicated that the organic substrates were oxidized incompletely to acetate; only Desulfobacter postgatei oxidized acetate with O2 completely to CO2. Many of the strains oxidized sulfite (completely to sulfate) or sulfide (incompletely, except Desulfobulbus propionicus); thiosulfate was oxidized only by strains of Desulfovibrio desulfuricans; trithionate and tetrathionate were not oxidized by any of the strains. With Desulfovibrio desulfuricans CSN and Desulfobulbus propionicus the oxidation of inorganic sulfur compounds was characterized in detail. D. desulfuricans formed sulfate during oxidation of sulfite, thiosulfate or elemental sulfur prepared from polysulfide. D. propionicus oxidized sulfite and sulfide to sulfate, and elemental sulfur mainly to thiosulfate. A novel pathway that couples the sulfur and nitrogen cycles was detected: D. desulfuricans and (only with nitrite) D. propionicus were able to completely oxidize sulfide coupled to the reduction of nitrate or nitrite to ammonia. Cell-free extracts of both strains did not oxidize sulfide or thiosulfate, but formed ATP during oxidation of sulfite (37 nmol per 100 nmol sulfite). This, and the effects of AMP, pyrophosphate and molybdate on sulfite oxidation, suggested that sulfate is formed via the (reversed) sulfate activation pathway (involving APS reductase and ATP sulfurylase). Thiosulfate oxidation with O2 probably required a reductive first step, since it was obtained only with energized intact cells.