Temporal dynamics of bacteria-plasmid coevolution under antibiotic selection.
Temporal dynamics of bacteria-plasmid coevolution under antibiotic selection.
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DOI：
10.1038/s41396-018-0276-9
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Brockhurst MA
中科院分区：
文献类型：
--
作者：
Bottery MJ;Wood AJ;Brockhurst MA
水平获得的基因即使编码有用的性状（如抗生素抗性），表达起来也可能代价高昂。我们以前表明，当选择四环素，大肠杆菌携带四环素耐药质粒RK 2进化突变的两个复制子，共同提供增加四环素耐药性，降低成本。在这里，我们调查的时间动态的基因组内的共同进化。使用基因组测序，我们表明，适应性突变的顺序是高度可重复的三个独立发展的人口。每个群体首先在ompF中获得染色体突变，从而缩短滞后期并增加四环素抗性。其次是突变损害质粒编码的四环素外排泵，最后，额外的耐药相关的染色体突变。因此，降低水平获得的四环素抗性的成本取决于首先进化一定程度的染色体编码抗性。因此，我们得出结论，细菌-质粒协同进化的轨迹被限制在一个单一的可重复的路径。
Horizontally acquired genes can be costly to express even if they encode useful traits, such as antibiotic resistance. We previously showed that when selected with tetracycline, Escherichia coli carrying the tetracycline-resistance plasmid RK2 evolved mutations on both replicons that together provided increased tetracycline resistance at reduced cost. Here we investigate the temporal dynamics of this intragenomic coevolution. Using genome sequencing we show that the order of adaptive mutations was highly repeatable across three independently evolving populations. Each population first gained a chromosomal mutation in ompF which shortened lag phase and increased tetracycline resistance. This was followed by mutations impairing the plasmid-encoded tetracycline efflux pump, and finally, additional resistance-associated chromosomal mutations. Thus, reducing the cost of the horizontally acquired tetracycline resistance was contingent on first evolving a degree of chromosomally encoded resistance. We conclude therefore that the trajectory of bacteria-plasmid coevolution was constrained to a single repeatable path.
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影响因子：
30.8
作者：
Toprak, Erdal;Veres, Adrian;Michel, Jean-Baptiste;Chait, Remy;Hartl, Daniel L.;Kishony, Roy
通讯作者：
Kishony, Roy
影响因子：
30.8
作者：
Manson AL;Cohen KA;Abeel T;Desjardins CA;Armstrong DT;Barry CE 3rd;Brand J;TBResist Global Genome Consortium;Chapman SB;Cho SN;Gabrielian A;Gomez J;Jodals AM;Joloba M;Jureen P;Lee JS;Malinga L;Maiga M;Nordenberg D;Noroc E;Romancenco E;Salazar A;Ssengooba W;Velayati AA;Winglee K;Zalutskaya A;Via LE;Cassell GH;Dorman SE;Ellner J;Farnia P;Galagan JE;Rosenthal A;Crudu V;Homorodean D;Hsueh PR;Narayanan S;Pym AS;Skrahina A;Swaminathan S;Van der Walt M;Alland D;Bishai WR;Cohen T;Hoffner S;Birren BW;Earl AM
通讯作者：
Earl AM
影响因子：
16.8
作者：
Bottery MJ;Wood AJ;Brockhurst MA
通讯作者：
Brockhurst MA
DOI：
10.1016/j.bbapap.2008.11.005
发表时间：
2009-05
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Delcour AH
通讯作者：
Delcour AH
影响因子：
4.9
作者：
Wang, H;Dzink-Fox, JL;Levy, SB
通讯作者：
Levy, SB