Functional analysis of circadian pacemaker neurons in Drosophila melanogaster
Functional analysis of circadian pacemaker neurons in Drosophila melanogaster
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DOI：
10.1523/jneurosci.1234-05.2006
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发表时间：
2006-03-01
影响因子：
5.3
通讯作者：
Helfrich-Förster, C
中科院分区：
文献类型：
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作者：
Rieger, D;Shafer, OT;Helfrich-Förster, C
昼夜节律的分子机制是众所周知的，但一个生物体内的多个时钟如何产生结构化的节律输出仍然是一个谜。许多动物显示出双峰活动节律，具有早晨（M）和晚上（E）活动发作。一个长期存在的模型假设，两个相互耦合的振荡器是这些发作的基础，并显示出不同的感光度。果蝇的行为节律由三组外侧神经元和三组背神经元控制。最近的数据表明，两组LN（腹侧亚组的小LN细胞和背侧亚组的LN细胞）是合理的候选人的M和E振荡器，分别。我们提供的证据表明，这些神经元群体对光的反应不同，并且可以通过恒定的光彼此完全去激活，导致两个活动组件以不同的周期自由运行。正如预期的那样，一个长周期的组成部分开始于E活动回合。然而，短周期分量不仅源于早高峰，更重要的是源于晚高峰。这揭示了一个有趣的偏离原来的Pittendridge和Daan（1976）的模型，并建议，一个亚组的腹侧子集的小LN作为“主”振荡器控制M和E活动的发作在果蝇。
The molecular mechanisms of circadian rhythms are well known, but how multiple clocks within one organism generate a structured rhythmic output remains a mystery. Many animals show bimodal activity rhythms with morning ( M) and evening ( E) activity bouts. One long-standing model assumes that two mutually coupled oscillators underlie these bouts and show different sensitivities to light. Three groups of lateral neurons (LN) and three groups of dorsal neurons govern behavioral rhythmicity of Drosophila. Recent data suggest that two groups of the LN ( the ventral subset of the small LN cells and the dorsal subset of LN cells) are plausible candidates for the M and E oscillator, respectively. We provide evidence that these neuronal groups respond differently to light and can be completely desynchronized from one another by constant light, leading to two activity components that free-run with different periods. As expected, a long-period component started from the E activity bout. However, a short-period component originated not exclusively from the morning peak but more prominently from the evening peak. This reveals an interesting deviation from the original Pittendrigh and Daan ( 1976) model and suggests that a subgroup of the ventral subset of the small LN acts as "main" oscillator controlling M and E activity bouts in Drosophila.