The effect of activin A on signal transduction pathways in PC12 cells subjected to oxygen and glucose deprivation
The effect of activin A on signal transduction pathways in PC12 cells subjected to oxygen and glucose deprivation
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DOI：
10.3892/ijmm.2013.1539
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发表时间：
2014-01-01
影响因子：
5.4
通讯作者：
Hu, Wenli
中科院分区：
文献类型：
--
作者：
Guo, Hongliang;Shen, Xiaoran;Hu, Wenli
The processes and mechanisms underlying brain injuries due to ischemia and anoxia have yet to be determined. Additionally, few clinical treatements are currently available. Activins have a protective role in the restoration, differentiation, and survival of injured cells, including Activin A (ActA), which acts as a neuroprotectant. However, its exact mechanism of action remains to be determined. ActA has been shown to protect neurons following ischemic brain injury. In this study, PC12 cells were differentiated into neuron-like cells after stimulation with nerve growth factor to prepare an oxygen/glucose deprivation (OGD) model in neurons. The differentiated PC12 cells, subjected to the OGD model, were exposed to ActA. Results showed that the PC12 survival rate decreased after OGD, leading to an increase in caspase-3 expression in these cells. Pretreatment with ActA was able to partially prevent OGD-induced apoptosis, likely through the downregulation of caspase-3. Futhermore, ActA pretreatment increased the expression of key proteins in the ActA/Smads signal transduction pathway, which may promote neuroprotection after OGD. Therefore, exogenous ActA may function as a neuroprotectant and provide a novel therapeutic treatment for ischemic brain injury.
