Isolation of tandem repeat sequences in the growth hormone gene of the red seabream, Pagrus major (Temminck & Schlegel, 1843)
Isolation of tandem repeat sequences in the growth hormone gene of the red seabream, Pagrus major (Temminck & Schlegel, 1843)
复制标题
红真鲷 (Temminck) 生长激素基因中串联重复序列的分离
DOI：
10.1111/jai.12800
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发表时间：
2015
影响因子：
0.9
通讯作者：
Motohiro Takagi
中科院分区：
文献类型：
--
作者：
Eitaro Sawayama;Motohiro Takagi
生长激素（GH）是出生后体细胞生长的主要调节因子之一，并刺激细胞分裂、骨骼生长和蛋白质合成等合成代谢过程（Goodman, 1993）。一些研究报道了生长激素基因作为提高家畜生长（Tambasco等人，2003）、抗病性、产蛋量（Kuhnlein等人，1997）、脂肪含量（Knorr等人，1997）和产奶量（Høj等人，1993;Dybus, 2002）等生产性状的候选基因。生长激素基因的多态性也被认为与一些水产养殖鱼类的一定程度的生长性能有关，如大西洋鲑鱼Salmo salar （Gross and Nilsson, 1999）、日本比目鱼parichthys olivaceus （Kang等，2002）和金头海鲷Sparus aurata （Almuly等，2005）。红海鲷（Pagrus major）是日本水产养殖业中重要的一种sparid鱼类（Murata, 2005）。几个孵化场成功培育出具有生长等有用经济性状的菌株（Murata et al., 1996; Taniguchi, 2011）。已知一些菌株具有除快速生长外的有用经济性状（Sawayama和Takagi， 2014），需要有效的方法来选择这些菌株的生长。因此，了解红鲷生长激素基因（pmaGH）的遗传结构及其串联重复序列的多态性对该鱼类未来的育种计划具有重要意义。在这项研究中，我们从红鲷中分离出生长激素基因（包括内含子和50 -侧翼区域的566 bp），并利用该物种野生种群的卫星DNA多态性来表征pmaGH的变异性。pmaGH的基因组结构也与一些鱼类生长激素基因进行了比较。
Growth hormone (GH) is one of the main regulators of postnatal somatic growth and stimulates anabolic processes such as cell division, skeletal growth and protein synthesis (Goodman, 1993). Several studies have reported the GH gene as a candidate for enhancing production traits such as growth (Tambasco et al., 2003), disease resistance, egg production (Kuhnlein et al., 1997), fat content (Knorr et al., 1997) and milk production (Høj et al., 1993; Dybus, 2002) in livestock. Polymorphisms of the GH gene are also known to be associated with some level of growth performance in several aquacultured fish species such as Atlantic salmon Salmo salar (Gross and Nilsson, 1999), Japanese flounder Paralichthys olivaceus (Kang et al., 2002) and gilthead seabream Sparus aurata (Almuly et al., 2005).Red seabream, Pagrus major is an important sparid fish species in the Japanese aquaculture industry (Murata, 2005). Several hatcheries successfully developed strains showing useful economic traits such as growth (Murata et al., 1996; Taniguchi, 2011). Some strains are known to possess useful economic traits other than fast growth (Sawayama and Takagi, 2014), and efficient methods are needed to select for growth in these strains. Therefore, understanding the genetic architecture of the GH gene of red seabream (pmaGH) and polymorphisms of tandem repeats located in pmaGH is important for future breeding programs of this fish species. In this study, we isolated the GH gene from red seabream (including introns and 566 bp of the 5 0-flanking region) and characterized the variability in pmaGH based on polymorphisms in the satellite DNA using a wild population of this species. The genomic structure of pmaGH was also compared with some fish GH genes.