Selective ablation of atheromas using a flashlamp-excited dye laser at 465 nm.
Selective ablation of atheromas using a flashlamp-excited dye laser at 465 nm.
复制标题
使用 465 nm 闪光灯激发染料激光选择性消融动脉粥样硬化。
DOI：
10.1073/pnas.83.18.7064
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发表时间：
1986
影响因子：
11.1
通讯作者：
Anderson,RR
中科院分区：
文献类型：
--
作者：
Prince,MR;Deutsch,TF;Shapiro,AH;Margolis,RJ;Oseroff,AR;Fallon,JT;Parrish,JA;Anderson,RR
在体外空气和生理盐水中，使用闪光灯激发的染料激光器在465 nm处的1 μ sec脉冲，用对正常动脉组织只有很小影响的激光脉冲消融人体动脉粥样硬化。在此波长下，由于类胡萝卜素，动脉粥样硬化中存在优先吸收。动脉粥样硬化消融的阈值能量密度为6.8 +/- 2.0 J/cm 2，正常主动脉组织消融的阈值能量密度为15.9 +/- 2.2 J/cm 2。在每脉冲18 J/cm 2的能量密度下，每单位能量的消融质量范围为161 - 370微克/J（动脉粥样硬化）和50 - 74微克/J（正常主动脉组织）。消融产物包括胆固醇晶体、胶原纤维碎片和少量钙化物质。大多数碎片的直径小于100微米，但少数碎片可达300微米。空气中烧蚀的高速摄影表明，由于蒸汽的形成，碎片以300米/秒的速度爆炸喷射。组织学分析显示对残留组织的热损伤极小。这些数据表明，选择性激光消融动脉粥样硬化是可能的，在体外。
Ablation of human atheromas with laser pulses that had only a small effect on normal artery tissue was shown in vitro in air and under saline using 1-mu sec pulses at 465 nm from a flashlamp-excited dye laser. At this wavelength, there is preferential absorption in atheromas due to carotenoids. The threshold fluence for ablation was 6.8 +/- 2.0 J/cm2 for atheromas and 15.9 +/- 2.2 J/cm2 for normal aorta tissue. At a fluence of 18 J/cm2 per pulse, the ablated mass per unit of energy ranged from 161 to 370 micrograms/J for atheromas and from 50 to 74 micrograms/J for normal aorta tissue. Ablation products consisted of cholesterol crystals, shredded collagen fibers, and small bits of calcific material. Most debris was less than 100 micron in diameter, but a few pieces were as large as 300 micron. High-speed photography of ablation in air suggested explosive ejection of debris, caused by vapor formation, at speeds on the scale of 300 m/sec. Histological analysis showed minimal thermal damage to residual tissue. These data indicate that selective laser ablation of atheromas is possible in vitro.