Luciferase a light source for the silica-based optical waveguides (spicules) in the demosponge Suberites domuncula
Luciferase a light source for the silica-based optical waveguides (spicules) in the demosponge Suberites domuncula
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DOI：
10.1007/s00018-008-8492-5
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发表时间：
2009-02-01
影响因子：
8
通讯作者：
Wiens, M.
中科院分区：
文献类型：
--
作者：
Mueller, W. E. G.;Kasueske, M.;Wiens, M.
两类海绵（动物门多孔动物）具有由骨针组成的硅质骨架。通过对六角海绵（> 5cm）大针状体的光学纤维机械性能的研究，证明了它们是有效的集光光学纤维。在这里，我们报告说，demosponge Suberites domuncula提供了一个生物传感器系统组成的（有机）光产生荧光素酶和（无机）光转换硅针状体。这些较小的针状体（200 μ m）的透光特性已得到证实，海绵组织产生光的能力也已得到证实。筛选S. domuncula的成功;制备了重组荧光素酶并显示出生物活性。荧光素酶蛋白大量存在于针状体附近。荧光素酶基因的表达受光的控制。
Two classes of sponges (animal phylum Porifera) possess a siliceous skeleton which is composed of spicules. Studying the optical fiber-mechanical properties of large spicules from hexactinellid sponges (> 5 cm) it was demonstrated that they are effective light-collecting optical fibers. Here, we report that the demosponge Suberites domuncula is provided with a biosensor system composed of the (organic) light producing luciferase and the (inorganic) light transducing silica spicules. The light transmission feature of these smaller spicules (200 mu m) has been demonstrated and the ability of sponge tissue to generate light has been proven. Screening for a luciferase gene in S. domuncula was successful; the recombinant luciferase was prepared and shown to be bioactive. The luciferase protein is abundantly present in the close neighborhood of the spicules. The expression of the luciferase gene is under the control of light.