Study on the toxic effects induced by different arsenicals in primary cultured rat astroglia
Study on the toxic effects induced by different arsenicals in primary cultured rat astroglia
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DOI：
10.1016/j.taap.2004.01.015
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发表时间：
2004-05-01
影响因子：
3.8
通讯作者：
Qu, L
中科院分区：
文献类型：
--
作者：
Jin, YP;Sun, GF;Qu, L
砷中毒是一个影响数百万人的全球性健康问题。本研究旨在探讨不同砷剂对原代培养大鼠星形胶质细胞的毒性作用。基于alamarBlue试验和单细胞凝胶电泳（SCGE，彗星试验），评价了不同砷剂暴露后细胞活力和DNA损伤。用甲基化砷剂，即五价单甲基胂酸（MMAV）和二甲基胂酸（DMAV）处理星形胶质细胞，在微摩尔浓度下细胞活力和DNA损伤没有明显变化。然而，治疗星形胶质细胞与无机砷，即亚砷酸盐和砷酸盐，导致细胞活力下降，增加DNA损伤的微摩尔水平，并显示出剂量相关的平均alamarBlue减少率和剂量相关的平均短号长度增加。因此，我们的研究高度提示无机暴露与细胞毒性或DNA损伤之间的联系。结果表明，亚砷酸盐的毒性作用强于砷酸盐。(C)2004年爱思唯尔公司All rights reserved.
Arsenic toxicity is a global health problem affecting millions of people. The objectives of this study were to determine if the toxic effects on primary cultured rat astroglia would be induced by different arsenicals. Based on alamarBlue assay and the single cell gel electrophoresis (SCGE, comet assay), the cell viability and DNA damage in the cells exposed to different arsenicals were evaluated. Treatment of astroglia with methylated arsenicals, that is, pentavalent monomethylarsonic acid (MMAV) and dimethylarsinic acid (DMAV), resulted in no obvious changes in cell viability and DNA damage at micromolar concentrations. However, treatment of astroglia with inorganic arsenicals, that is, arsenite and arsenate, caused decreased cell viability and increased DNA damage at micromolar levels, and showing a dose-related decrease in mean alamarBlue reduced rate and a dose-related increase in mean cornet length. Our study is therefore highly suggestive for a link between inorganic exposure and cellular toxicity or DNA damage. Based on the results of this study, the toxic effects induced by arsenite were stronger than those induced by arsenate. (C) 2004 Elsevier Inc. All rights reserved.