Identification of mRNA for endocannabinoid biosynthetic enzymes within hippocampal pyramidal cells and CA1 stratum radiatum interneuron subtypes using quantitative real-time polymerase chain reaction.
Identification of mRNA for endocannabinoid biosynthetic enzymes within hippocampal pyramidal cells and CA1 stratum radiatum interneuron subtypes using quantitative real-time polymerase chain reaction.
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DOI：
10.1016/j.neuroscience.2012.05.012
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发表时间：
2012-08-30
期刊：
影响因子：
3.3
通讯作者：
Edwards, J. G.
中科院分区：
文献类型：
--
作者：
Merrill, C. B.;McNeil, M.;Williamson, R. C.;Poole, B. R.;Nelson, B.;Sudweeks, S.;Edwards, J. G.
海马是短期记忆所必需的，并且包含兴奋性锥体细胞和抑制性中间神经元。这些细胞表现出各种形式的突触可塑性，学习和记忆的基础机制。最近，内源性大麻素被确定参与突触可塑性。我们的目标是描述内源性大麻素生物合成酶的分布在CA 1层辐射中间神经元和CA 3/CA 1锥体细胞。我们从单个中间神经元和锥体细胞中提取mRNA，并使用实时定量PCR检测12-脂氧合酶，N-酰基-磷脂酰乙醇胺特异性磷脂酶D，二酰基甘油脂酶和I型代谢型谷氨酸受体的存在，已知参与内源性大麻素的产生和可塑性。我们观察到内源性大麻素生物合成酶mRNA的表达确实发生在中间神经元内，并且它与I型代谢型谷氨酸受体共表达，这表明中间神经元具有产生内源性大麻素的潜力。我们还发现，CA 3和CA 1锥体细胞表达内源性大麻素生物合成酶mRNA。我们的数据提供了第一个假定的内源性大麻素在中间神经元生产的分子生物学证据，表明他们的潜在能力，调节内源性大麻素介导的过程，如突触可塑性。
The hippocampus is required for short-term memory and contains both excitatory pyramidal cells and inhibitory interneurons. These cells exhibit various forms of synaptic plasticity, the mechanism underlying learning and memory. More recently, endocannabinoids were identified to be involved in synaptic plasticity. Our goal was to describe the distribution of endocannabinoid biosynthetic enzymes within CA1 stratum radiatum interneurons and CA3/CA1 pyramidal cells. We extracted mRNA from single interneurons and pyramidal cells and used real-time quantitative PCR to detect the presence of 12-lipoxygenase, N-acyl-phosphatidylethanolamine-specific phospholipase D, diacylglycerol lipase, and type I metabotropic glutamate receptors, known to be involved in endocannabinoid production and plasticity. We observed that the expression of endocannabinoid biosynthetic enzyme mRNA does occur within interneurons and that it is coexpressed with type I metabotropic glutamate receptors, suggesting interneurons have the potential to produce endocannabinoids. We also identified that CA3 and CA1 pyramidal cells express endocannabinoid biosynthetic enzyme mRNA. Our data provide the first molecular biological evidence for putative endocannabinoid production in interneurons, suggesting their potential ability to regulate endocannabinoid-mediated processes, such as synaptic plasticity.
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DOI：
10.1523/jneurosci.6451-10.2011
发表时间：
2011-03-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cavanaugh DJ;Chesler AT;Jackson AC;Sigal YM;Yamanaka H;Grant R;O'Donnell D;Nicoll RA;Shah NM;Julius D;Basbaum AI
通讯作者：
Basbaum AI
DOI：
10.1073/pnas.89.10.4363
发表时间：
1992-05-15
影响因子：
11.1
作者：
DUDEK, SM;BEAR, MF
通讯作者：
BEAR, MF
影响因子：
15.9
作者：
ALGER, BE;PITLER, TA
通讯作者：
PITLER, TA
影响因子：
3.3
作者：
Cristino, L.;De Petrocellis, L.;Di Marzo, V.
通讯作者：
Di Marzo, V.
影响因子：
5.5
作者：
Gee, CE;Benquet, P;Gerber, U
通讯作者：
Gerber, U