Kinetic study of all-or-none hemolysis induced by catonic amphiphilic polymethacrylates with antimicorbial activity
Kinetic study of all-or-none hemolysis induced by catonic amphiphilic polymethacrylates with antimicorbial activity
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具有抗菌活性的阳离子两亲性聚甲基丙烯酸酯引起的全或无溶血的动力学研究
DOI：
10.1016/j.cclet.2015.01.029
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发表时间：
2015
影响因子：
9.1
通讯作者：
Kenichi Kuroda
中科院分区：
文献类型：
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作者：
Kazuma Yasuhara;Kenichi Kuroda
为了了解抗菌聚合物对人体细胞的毒性，用人红细胞(RBC)研究了它们的溶血作用。我们研究了以氨乙基侧链为阳离子单元，以甲基丙烯酸丁酯或甲基丙烯酸甲酯为疏水单元的阳离子两亲性甲基丙烯酸酯无规共聚物的溶血作用。含有30%丁基侧链的聚合物(B30)的溶血毒性高于含有59%甲基侧链的聚合物(M59)。B30也比M59更快地诱导红细胞释放血红蛋白。提出了一个新的理论模型，该模型基于连续两步在红细胞膜上形成与红细胞裂解相关的活性聚合物物种。这个模型考虑了红细胞破裂时全有或全无释放血红蛋白的情况，为了解聚合物诱导的溶血提供了关于单个或集体细胞如何对聚合物做出反应的新见解。
To gain an understanding of the toxicity of antimicrobial polymers to human cells, their hemolytic action was investigated using human red blood cells (RBCs). We examined the hemolysis induced by cationic amphiphilic methacrylate random copolymers, which have amino ethyl sidechains as cationic units and either butyl or methyl methacrylate as hydrophobic units. The polymer with 30 mol% butyl sidechains (B30) displayed higher hemolytic toxicity than the polymer with 59 mol% methyl sidechains (M59).B30also induced faster release of hemoglobin from RBCs thanM59. A new theoretical model is proposed based on two consecutive steps to form active polymer species on the RBC membranes, which are associated to RBC lysis. This model takes the all-or-none release of hemoglobin by the rupture of RBCs into account, providing new insight into the polymer-induced hemolysis regarding how individual or collective cells respond to the polymers.