Improved fluorometric method for quantitative high-performance liquid chromatographic analysis of methylated guanine derivatives in DNA.
Improved fluorometric method for quantitative high-performance liquid chromatographic analysis of methylated guanine derivatives in DNA.
复制标题
改进的荧光方法，用于 DNA 中甲基化鸟嘌呤衍生物的定量高效液相色谱分析。
DOI：
10.1016/s0021-9673(01)81353-4
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Pezzuto,JM
中科院分区：
文献类型：
--
作者：
Ratko,TA;Pezzuto,JM
使用Waters高效液相色谱系统，包括M720型溶剂输送系统，配备M6OOOA型和M45型泵，WISP 710B型自动进样器和M730型数据缩减和文档模块。荧光监测使用Gilson Medical Electronics (Middleton， WI， USA) 121型在线荧光检测器，配备280 nm波长激发电源、灯和滤光片。彩色玻璃发射滤光片来自康宁，通带为350470nm。
A Waters HPLC system comprised of a Model M720 solvent delivery system equipped with Model M6OOOA and Model M45 pumps, a WISP 710B automatic sample injector and Model M730 data reduction and documentation module was used. Fluorescence was monitored using a Gilson Medical Electronics (Middleton, WI, USA) Model 121 on-line fluorescence detector equipped with a 280-nm wavelength excitation power source, lamp and filter. The colored-glass emission filter was from Corning, with a 350470 nm passband.