HUMAN LYMPHOCYTE SUBPOPULATIONS - EFFECT OF CORTICOSTEROIDS
HUMAN LYMPHOCYTE SUBPOPULATIONS - EFFECT OF CORTICOSTEROIDS
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DOI：
10.1172/jci107591
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发表时间：
1974-01-01
影响因子：
15.9
通讯作者：
BARNETT, EV
中科院分区：
文献类型：
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作者：
YU, DTY;CLEMENTS, PJ;BARNETT, EV
正常受试者在上午8点口服泼尼松60毫克。在下午2点出现一过性淋巴细胞减少症。检测外周血中淋巴细胞的数量和类型，以确定受影响的淋巴细胞群体。“晚期”和“早期”自发绵羊红细胞花环分别作为胸腺来源(T)淋巴细胞及其一个亚群的标记。聚集的丙种球蛋白和补体的受体分别识别法氏囊或骨髓来源的(B)淋巴细胞及其一个亚群。服用60 mg强的松后6h，T细胞比例从69.2+/-2.1%下降到55.9+/-2.8%(平均+/-SE)，B细胞比例从21.3+/-2.0%上升到44.8+/-4.1%。“早期”花环和补体受体淋巴细胞的变化也与此相平行。在所有病例中，强的松均使T细胞和B细胞的绝对数减少。强的松治疗后淋巴细胞密度梯度分布无明显变化。这些数据表明，强的松对T和B淋巴细胞均有影响，但T细胞淋巴细胞减少更为明显。淋巴细胞减少可能反映了骨髓中的隔离和/或短暂的再循环停止。
Normal subjects given 60 mg of prednisone orally at 8:00 a.m. developed a transient lymphopenia at 2:00 p.m. To define the populations of lymphocytes affected the number and type of lymphocytes in the peripheral blood were assayed. "Late" and "early" spontaneous sheep red blood cell rosettes were used as markers for thymus-derived (T) lymphocytes and one of its subpopulations, respectively. Receptors for aggregated gammaglobulin and complement identified bursal-equivalent or bone marrow-derived (B) lymphocytes and one of its subpopulations, respectively. 6 h after administration of 60 mg of prednisone, the blood samples showed a decrease in proportion of T cells from 69.2 +/- 2.1% to 55.9 +/- 2.8% (average +/- SE) and an increase in B-cell proportion from 21.3 +/- 2.0% to 44.8 +/- 4.1%. The changes of "early" rosettes and complement receptor lymphocytes also paralleled these. In all cases the absolute numbers of T cells and of B cells were decreased by prednisone. The density gradient distribution of the lymphocytes did not change after prednisone. These data indicate that both T and B lymphocytes are affected by the prednisone but that the T cell lymphopenia was more pronounced. The lymphopenia might reflect either sequestration in the marrow and/or transient arrest of recirculation.