Purification of Exfoliatin Produced by Staphylococcus aureus of Bacteriophage Group 2 and Its Physicochemical Properties
Purification of Exfoliatin Produced by Staphylococcus aureus of Bacteriophage Group 2 and Its Physicochemical Properties
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噬菌体2组金黄色葡萄球菌去角质素的纯化及其理化性质
DOI：
10.1128/iai.8.2.156-164.1973
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发表时间：
1973
影响因子：
3.1
通讯作者：
Y. Sarai
中科院分区：
文献类型：
--
作者：
I. Kondo;S. Sakurai;Y. Sarai
Melish等人最近报道了一种有效的分离和纯化去角质素的方法，该去角质素是导致烫伤皮肤综合征的葡萄球菌毒素，并描述了纯化毒素的理化性质。从噬菌体组 2 的临床分离株之一产生的活性粗毒素中，获得了四种类型的有毒蛋白质，它们都能够在新生小鼠中引起典型的 Nikolsky 征，并将其命名为 A、B、C 和 D 毒素。它们的分子量约为24,000，在中和和沉淀试验中表现出相同的血清学特征，但彼此不同，在聚丙烯酰胺盘电泳中显示不同的单条带及其各自的迁移率。它们在 pH 值 4.0 至 4.5 之间沉淀并失去剥落能力。然而，产生的沉淀物可以溶解在含有 0.5% 十二烷基硫酸钠的乙酸盐缓冲液中，将其毒性恢复到几乎与以前相同的程度。它们在60℃加热60分钟和100℃加热20分钟时均稳定，但在100℃加热40分钟时失去毒性。此外，本作者观察到，从烫伤皮肤综合征患者中分离出的一些不属于典型噬菌体 2 组的葡萄球菌菌株也能够产生类似但血清学上无关的剥脱性毒素。
An effective method for the isolation and purification of exfoliatin which has been recently reported by Melish and others as the staphylococcal toxin responsible for the scalded skin syndrome and the physicochemical properties of the purified toxin were described. From an active crude toxin produced by one of the clinical isolates of phage group 2, four types of toxic proteins which were all capable of causing the typical Nikolsky sign in neonatal mice were obtained and designated A, B, C, and D toxins. They had a molecular weight of about 24,000 and showed the same serological features in neutralization and precipitation tests, but were different from each other in showing a different single band with their respective mobilities in polyacrylamide disk electrophoresis. They were precipitated between pH 4.0 and 4.5 and lost their exfoliative capabilities. The resulting precipitates, however, could be solubilized in acetate buffer containing 0.5% sodium dodecyl sulfate, restoring their toxicities to almost the same extent as before. They were all stable when heated at 60 C for 60 min and at 100 C for 20 min, but lost their toxicities when heated at 100 C for 40 min. Additionally, the present authors observed that some staphylococcal strains not belonging to the typical phage group 2, isolated from patients with the scalded skin syndrome, were also capable of producing a similar but serologically unrelated exfoliative toxin.