A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model
A Novel Alaska Pollock Gelatin Sealant Shows Higher Bonding Strength and Nerve Regeneration Comparable to That of Fibrin Sealant in a Cadaveric Model and a Rat Model
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新型阿拉斯加狭鳕明胶密封剂在尸体模型和大鼠模型中显示出与纤维蛋白密封剂相当的更高的粘合强度和神经再生能力
DOI：
10.1097/prs.0000000000008489
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发表时间：
2021
影响因子：
3.6
通讯作者：
Masaya Nakamura
中科院分区：
文献类型：
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作者：
Shusuke Masuda;Taku Suzuki;Shinsuke Shibata;Nobuko Moritoki;Yoshifumi Abe;Xi Chen;Yosuke Mizuno;Akihiro Nishiguchi;Hiroo Kimura;Noboru Matsumura;Takuji Iwamoto;Tetsushi Taguchi; Morio Matsumoto; Masaya Nakamura
背景：一种由阿拉斯加鳕鱼衍生明胶（ApGltn）组成的新型生物相容性密封剂最近在猪主动脉中显示出良好的爆破强度和生物相容性。本研究的目的是调查的粘合强度和ApGltn密封剂在新鲜冷冻尸体和大鼠模型的坐骨神经横断的指神经的生物力学牵引测试。方法：80人新鲜冷冻尸体的指神经横断。采用四种手术干预进行治疗：（1）缝线+ApGltn封闭剂;（2）缝线;（3）ApGltn封闭剂;和（4）纤维蛋白封闭剂。43个坐骨神经的雄性Wistar大鼠用于功能和组织病理学评价。采用6种手术干预进行治疗：（1）缝线+ApGltn封闭剂;（2）缝线;（3）ApGltn封闭剂;（4）纤维蛋白封闭剂;（5）切除5 mm间隙（每组10只大鼠）;（6）假手术（3只大鼠）。结果：ApGltn封闭剂的最大破坏载荷显著高于纤维蛋白封闭剂（0.22±0.05 N vs 0.06±0.04 N）。ApGltn密封剂的最大失效载荷显著低于缝线+ApGltn密封剂（1.37 N）和缝线（1.27 N）。结论：与纤维蛋白封闭剂相比，ApGltn封闭剂具有更高的粘接强度和相同的神经再生效果。
Background:A novel biocompatible sealant composed of Alaska pollock–derived gelatin (ApGltn) has recently shown good burst strength and biocompatibility in a porcine aorta. The purpose of this study was to investigate the bonding strength and biocompatibility of the ApGltn sealant in transected digital nerves of fresh frozen cadavers and in the sciatic nerves of a rat model.Methods:Eighty human digital nerves of fresh frozen cadavers were transected for biomechanical traction testing. They were treated with four surgical interventions:(1) suture plus ApGltn sealant;(2) suture;(3) ApGltn sealant; and (4) fibrin sealant. Forty-three sciatic nerves of male Wistar rats were used for functional and histopathologic evaluation. They were treated with six surgical interventions:(1) suture plus ApGltn sealant;(2) suture;(3) ApGltn sealant;(4) fibrin sealant;(5) resection with a 5-mm gap (10 rats per group); and (6) sham operation (three rats). Macroscopic confirmation, muscle weight measurement, and histopathologic findings including G-ratio were examined 8 weeks after the procedure.Results:The maximum failure load of the ApGltn sealant was significantly higher than that of a fibrin sealant (0.22±0.05 N versus 0.06±0.04 N). The maximum failure load of the ApGltn sealant was significantly lower that of suture plus ApGltn sealant (1.37 N) and suture (1.27 N). Functional evaluation and histologic examination showed that sciatic nerves repaired with ApGltn sealant showed similar nerve recovery compared to repair with the suture and fibrin sealant.Conclusion:The ApGltn sealant showed higher bonding strength and equal effect of nerve regeneration when compared with the fibrin sealant.